23. Stent, G.S. (1972). Prematurity and uniqueness in scientific discovery. Sci. Am. 227, 84–93. 24. Mirsky, A.E., and Pollister, A.W. (1946). Chromosin, a desoyribose nucleoprotein complex of the cell nucleus. J. Gen. Physiol. 30, 117–148. 25. Boivin, A. (1947). Directed mutation in colon bacilli, by an inducing principle of desoxyribonucleic nature: Its meaning for the general biochemistry of...

This study examined the effects of cold-water immersion (CWI) and cold air therapy (CAT) on maximal cycling performance (i.e. anaerobic power) and markers of muscle damage following a strength training session. Twenty endurance-trained but strength-untrained male (n = 10) and female (n = 10) participants were randomised into either: CWI (15 min in 14 °C water to iliac crest) or CAT (15 min in 1...

References 1 Deming, J.W. (2002) Psychrophiles and polar regions. Curr. Opin. Microbiol. 5, 301–309 2 Deming, J.W. and Eicken, H. (2004) Life in ice. In Planets and Life: The Emerging Science of Astrobiology (Baross, J.A. and Sullivan, W., eds), Cambridge University Press (in press) 3 Bowman, J.P. et al. (1997) Diversity and association of psychrophilic bacteria in antarctic sea ice. Appl. Envi...

Isoelectric Focusing in Immobilized pH Gradient Strips Using the IPGphor Unit: Sample In-Gel Rehydration Angelika Görg, Oliver Drews, and Walter Weiss This protocol was adapted from "IEF in IPG Strips Using the IPGphor Unit," contributed by Angelika Görg, Oliver Drews, and Walter Weiss, Chapter 16, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spr...

Denaturing Isoelectric Focusing Free-Flow Electrophoresis (IEF-FFE) Fractionation of Crude Protein Mixtures Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn This protocol was adapted from "Denaturing IEF-FFE Fractionation of Crude Protein Mixtures," contributed by Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn, Chapter 19, in Purifying Proteins for Proteomics (ed. Simpson). ...

Protocol A Magnetic Particle-Based Method for Purifying PCR Products from Solution Craig Smith, Paul Otto, Rex Bitner, and Gary Shiels This protocol was adapted from "DNA Purification," contributed by Craig Smith, Paul Otto, Rex Bitner, and Gary Shiels in Chapter 9, PCR Primer, 2nd edition (eds. Dieffenbach and Dveksler). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA, 2...

Native Isoelectric Focusing Free-flow Electrophoresis (IEF-FFE) Fractionation of Crude Protein Mixtures Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn This protocol was adapted from "Native IEF-FFE Fractionation of Crude Protein Mixtures," contributed by Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn, Chapter 19, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spr...

The study focused on the possibilities of evaluation red pepper (Capsicum Annuum L.) seeds being a food industry waste. moisture content (%), total crude oil and ash content, color, weight 1000 seeds, thickness diameter were characterized. oils extracted from using green techniques: cold-pressing, ethanol solvent, ultrasound-assisted solvent extraction.Different techniques compared production y...