Introduction: Based on the evidence, the Pedunculopontine Tegmental nucleus (PPT) is involved in cardiovascular function regulation. In this study, the probable role of PPT on cardiovascular parameters in the hypotension induced by Hemorrhage (HEM) was evaluated.  Methods: The study rats were divided up into 5 groups: 1. Control (Saline); 2. Cobalt(II) chloride (CoCl2); 3. HEM; 4. Saline+HEM; ...

Aim. We investigated whether lecithin-bound iodine (LBI) can protect the integrity of tight junctions of retinal pigment epithelial cells from hypoxia. Method. Cultured human retinal pigment epithelial (ARPE-19) cells were pretreated with LBI. To mimic hypoxic conditions, cells were incubated with CoCl2. We compared the integrity of the tight junctions (TJs) of control to cells with either LBI ...

BACKGROUND Perioperative cerebral ischemia/hypoxia could induce hippocampal injury and has been reported to induce cognitive impairment. In this study, we used cobalt chloride (CoCl2) to build a hypoxia model in mouse hippocampal cell lines. Propofol, a widely used intravenous anesthetic agent, has been demonstrated to have neuroprotective effect. Here, we explored whether and how propofol atte...

Heme oxygenase (HO)-1 expression was investigated in rat isolated pancreatic islets. Freshly isolated islets showed no evidence of HO-1 expression. After a 20-h culture, there was a small increase in HO-1 in control islets, and interleukin-1beta (IL-1beta) induced HO-1 expression above control levels. N(G)-monomethyl-L-arginine inhibited the IL-1beta-induced increase in HO-1. Sodium nitroprussi...

Purpose: To establish epithelial-mesenchymal transition (EMT) models in renal cell carcinoma cell lines by CoCl2-induced hypoxia. Materials and methods: The renal cell carcinoma cell lines A498 and 786-O were used in the experiment and CoCl2 was used to simulate hypoxia. The cancer cells were cultured with different concentrations of CoCl2. Morphology and cyto-activity changes were detected to ...

Cobalt chloride (CoCl2) modifies mitochondrial permeability and has a hypoxic-mimetic effect; thus, the compound induces tolerance to ischemia and increases resistance to a number of injury types. The aim of the present study was to investigate the effects of CoCl2 hypoxic preconditioning for three weeks on thermonociception, somatic and visceral inflammatory pain, locomotor activity and coordi...

Purpose: Cobalt-chloride (CoCl2) induces a strong degeneration of the porcine retina through hypoxic mechanisms. Neurons of the inner retina layers are particularly affected. This model is used to study possible protective effects of hypothermia treatment. Methods: Organotypic cultures of porcine retinas were cultured and treated with 300 μM CoCl2 for 48 h. Hypothermia (30°C) was applied for th...

Mesenchymal stem cells (MSCs) in the bone marrow and other somatic tissues reside in an environment with relative low oxygen tension. Cobalt chloride (CoCl2) can mimic hypoxic conditions through transcriptional changes of some genes including hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF). This study evaluated the potential role of CoCl2 preconditioning on mu...

To explore the cellular adaptations and responses to hypoxia in normal human saphenous vein smooth muscle cells (SMCs) and presume what roles phenotypic modulation of normal human saphenous vein SMCs would play in varicose vein of lower extremity, we used cobalt chloride (CoCl2), a hypoxia mimetic, to treat normal human saphenous vein SMCs in vitro. The proliferating ability of cells exposed to...

Retinal ganglion cells (RGCs) consume large quantities of energy to convert light information into a neuronal signal, which makes them highly susceptible to hypoxic injury. This study aimed to investigate the potential protection by baclofen, a GABAB receptor agonist of RGCs against hypoxia-induced apoptosis. Cobalt chloride (CoCl2) was applied to mimic hypoxia. Primary rat RGCs were subjected ...