The enzyme L-Amino Acid Oxidase (LAAO) is widely distributed in snake venoms, contributes a reasonable toxicity to the venoms. However LAAO from Indian cobra (Naja naja ) snake venom has not yet isolated previously. In the present study LAAO from Indian cobra (Naja naja ) snake venom was purified by sequential steps of cation exchange chromatography (CM-Sephadex C-25; CM-52 cellulose), followed...

Mucuna pruriens Linn. (velvet bean) has been used by native Nigerians as a prophylactic for snakebite. Rats pretreated with M. pruriens seed extract (MPE) have been shown to protect against the lethal and cardiovascular depressant effects of Naja sputatrix (Javan spitting cobra) venoms, and the protective effect involved immunological neutralization of the venom toxins. To investigate further t...

A high molecular weight, heavily glycosylated protein fraction was isolated from cobra venom. It consists of mucin-like glycoproteins (designated as cobra venom mucin) in noncovalent association with several lower molecular weight proteins and glycoproteins. The mucin was purified by CsBr density gradient centrifugation under dissociative conditions. The purified venom mucin comprised about 85%...

Crotoxin, the first crystalline protein from snake venom isolated by Slotta and Fraenkel-Conrat (1) in 1938 from the venom of Crotalus terr$cus terr$icus, carried both neurotoxic and hemolytic activities. However, it was found later that there were at least two markedly different proteins in this crystalline preparation (2). In 1944, De (3) isolated crystalline hemolysin from the venom of Naja ...

The venom of Naja naja has been studied clinically as an analgesic. It has been recently determined that the venom of cobra is biologically active even when it is administered by mouth. As there are no published studies on the effects of orally administered cobra venom, a 72h toxicity study was conducted in the Albino rats. We have selected 1/50 LD50 dose (7μg/ kg body weight) in the present in...

BACKGROUND Patients with chronic pain usually suffer from cognitive impairment, with memory deterioration being the most common deficit that affects daily functioning and quality of life. The causes for this impairment are not clear despite intensive clinical studies. Few studies have evaluated impaired learning using animal models of persistent pain. OBJECTIVE In this study, a new trigeminal...

PURPOSE To report the first case of ophthalmia due to contact with cobra venom in the Volta Region, Ghana. METHODS An ointment containing vitamin A was applied to treat the patient's unilateral defects in the corneal epithelium and the consequent diminished visual acuity. RESULTS Healing of the corneal epithelium and improvement of visual acuity were observed after only 1 day. CONCLUSIONS...

Acetylcholinesterase purified from cobra (Naja naja) venom exhibits a serotonin-sensitive aryl acylamidase activity. Both acetylcholinesterase and aryl acylamidase activities co-eluted in column chromatographic procedures (Sephadex G-75 and Zinc-Sepharose), co-migrated on polyacrylamide gel electrophoresis, co-immunoprecipitated by anti-snake venom antibody and showed the same heat denaturation...

The role of the complement system in containment of feline leukemia virus infection was studied by cobra venom factor treatment of feline leukemia virus-immune cats. One to three weeks after cobra venom factor treatment, an increase in viral antigen in marrow myelomonocytic cells and circulating immune complexes was noted. Prevention of reactivation of feline leukemia virus infection may in par...