BACKGROUND The gold standard for the diagnosis of schistosomiasis is the detection of the parasite's characteristic eggs in urine, stool, or rectal and bladder biopsy specimens. Direct detection of eggs is difficult and not always possible in patients with low egg-shedding rates. Confocal laser scanning microscopy (CLSM) permits non-invasive cell imaging in vivo and is an established way of obt...

The use of immunohistochemical detection techniques and fluorescent molecular probes in light and fluorescence microscopy allows accurate and specific analysis of a great variety of cell and tissue components. However, when staining yields only low intensity levels, serious problems may arise with discrimination of specific signals against background staining. This problem is often inherent wit...

The article reviews the existing methods of multicolor FISH on nuclear targets, first of all, interphase chromosomes. FISH proper and image acquisition are considered as two related components of a single process. We discuss (1) M-FISH (combinatorial labeling + deconvolution + wide-field microscopy); (2) multicolor labeling + SIM (structured illumination microscopy); (3) the standard approach t...

An investigation was conducted to determine the influence of recycled scrap tire rubber on the engineering properties of controlled low strength material (CLSM). With control over the ultimate compressive strength of CLSMs being key for applications such as backfilling utility trenches where re-excavation is often necessary, incorporation of scrap tire rubber into the CLSM mixture is a viable w...

An investigation was conducted to determine the influence of recycled scrap tire rubber on the engineering properties of controlled low strength material (CLSM). With control over the ultimate compressive strength of CLSMs being key for applications such as backfilling utility trenches where re-excavation is often necessary, incorporation of scrap tire rubber into the CLSM mixture is a viable w...

Confocal laser scanning microscopy (CLSM) is a modern powerful technique that can be used for studying the external and internal anatomy of arthropods. CLSM has seldom been used in acarology and very rarely for studying eriophyoid mites. It allows the capture of precise digital images of the fine details of external and internal chitinous structures, which can be further analysed using various ...

In view of the increasing concerns over non-renewable resource depletion and waste management, this paper studied development low-density controlled low-strength material (CLSM) by using stone sludge lightweight aggregates. First, investigation was performed at a laboratory scale to assess effects composition on properties resulting CLSM. The Taguchi method with an L9(34) orthogonal array four ...

BACKGROUND The confocal laser scanning microscope (CLSM) has been used by scientists to visualize three-dimensional (3D) biological samples. Although this system involves lasers, electronics, optics, and microscopes, there are few published tests that can be used to assess the performance of this equipment. Usually the CLSM is assessed by subjectively evaluating a biological/histological test s...

Objective: Otitis media with effusion (OME), a common chronic childhood condition affecting hearing, is thought to be a result of bacterial infection, with biofilms recently implicated. Although bacterial DNA can be detected by polymerase chain reaction in 80% of patients, typically fewer than half of effusions are positive using standard culture techniques. We adopted an alternative approach t...