Nine Clostridium perfringens strains were isolated from fifteen intestinal content samples collected from commercial broilers which suffered from necrotic enteritis NE. Molecular typing of C. perfringens was performed by PCR using three sets of primers specific for toxin -producing genes of C. perfringens [alpha (900bp), beta (236bp) and epsilon (541bp)]. Six strains of C. perfringens type A an...

Ellner, Paul D. (University of Vermont, Burlington) and Carolyn D. Bohan. Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion. J. Bacteriol. 83:284-296. 1962.-A serological study by agar-gel diffusion of the soluble antigens of 39 strains of the Clostridium perfringens group has shown them to be extremely heterogeneous. Strain variation occurred within th...

NanI Sialidase, CcpA and CodY Work Together to Regulate Epsilon Toxin 1 Production by Clostridium perfringens Type D Strain CN3718 2 3 Jihong Li, John C. Freedman and Bruce A. McClane* 4 Department of Microbiology and Molecular Genetics, University of Pittsburgh School of 5 Medicine, Pittsburgh, PA, USA 15219 6 7 8 Running title: C. perfringens NanI regulates ETX production 9

Toxigenic types of Clostridium perfringens are significant causative agents of enteric disease in domestic animals, although type E is presumably rare, appearing as an uncommon cause of enterotoxemia of lambs, calves and rabbits. We report herein the typing of 23 C. perfringens strains, by the polymerase chain reaction (PCR) technique, isolated from small intestine samples of bovines that have ...

Aim: To identify the Clostridium perfringens toxin genotypes prevailing in enterotoxemia suspected sheep flocks in Andhra Pradesh by using multiplex PCR. Materials and Methods: Intestinal scrapings were collected from lambs below three months of age from flocks with similar management from different Districts of Andhra Pradesh, in India. A total of 208 samples were collected with 140 from enter...

The presence and molecular characterization of Clostridium perfringens in healthy Merino lambs over a six-month period was investigated in this study. Overall, a high prevalence of C. perfringens was detected, even in day-old lambs. Even though the majority of the isolates were characterized as being of type A, types C and D were also isolated. Furthermore, a high genetic diversity was observed...

s: Dennison A, Plampin E, Ellis R, Van Metre D, et al (2001). Correlation of Clostridium perfringens a toxin activity and disease severity in isolates from cows with hemorrhagic bowel syndrome (abstract). Proceedings, 82nd Annual Meeting, Conference of Research Workers in Animal Diseases, 108. Dennison A, Van Metre D, Callan R, Mason G, et al (2002). Hemorrhagic bowel syndrome of adult cattle; ...

Clostridium perfringens (C. perfringens) is an important cause of bacterial food poisoning worldwide. The disease is caused by C. perfringens enterotoxin (CPE) encoded by cpe gene. The aim of this research was to identify the different types of C. perfringens and the presence of cpe gene in isolated bacteria from broilers' meat marketed in retail meat shops of Mashhad city in Northeastern of Ir...