Bacterial colony enumeration is an essential tool for many widely used biomedical assays. This chapter introduces a cost-effective and fully automatic bacterial colony counter which accepts digital images as its input. The proposed counter can handle variously shaped dishes/plates, recognize chromatic and achromatic images, and process both color and clear medium. In particular, the counter can...

Clonogenic assays are the gold standard for determining radiosensitivity, which governs tumor response to radiation therapy. Although multiple studies of clonogenic assays on cancer cell lines have been published, the robustness of this technique has not been examined by comparative analysis of data from different studies. To address this issue, we investigated the inter-assay precision of clon...

PURPOSE Reliable in vitro cytotoxicity assays are essential for determining the responses of human normal and cancer-derived cells to therapeutic agents and also for the identification and pre-clinical evaluation of new drugs capable of selectively augmenting the susceptibility of cancer cells to conventional therapies. The clonogenic survival assay is considered as the "gold standard" in this ...

Drug sensitivity assays were performed using a variation of a colori metrie [3-{4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)1 assay on V79, CHO-AuxBl, CH"C5, NCI-H460, and NCIH249 cell lines following optimization of experimental conditions for each cell line. Results from this assay were compared with data assimi lated simultaneously by clonogenic assay and by dye exclusion ...

Drug sensitivity assays were performed using a variation of a colori metrie [3-{4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)1 assay on V79, CHO-AuxBl, CH"C5, NCI-H460, and NCIH249 cell lines following optimization of experimental conditions for each cell line. Results from this assay were compared with data assimi lated simultaneously by clonogenic assay and by dye exclusion ...

Abstract Background Gold nanoparticles (GNPs) have been used to sensitize cancer cells and enhance the absorbed dose delivered such cells. Active targeting can provide specific effect higher uptake of GNPs in tumor cells, while having small on healthy The aim this study was assess possible radiosensitiazation conjugated with AS1411 aptamer (AS1411/GNPs) treated 4 MeV electron beams. Materials m...

Background:  Cell viability is an important factor in radiation therapy and thus is a method to quantify the effect of the therapy. Materials and Methods: The viability of human hepatoma (HepG2) cells exposed to radiation was evaluated by both the MTT and Trypan blue assays. The cells were seeded on 96 well-plates at a density of 1 x 104 cells/well, incubated overnight, and irradiated with...