introduction: quantification of epstein - barr virus (ebv) in peripheral blood mononuclear cells (pbmnc) of allogenic bone marrow transplant (bmt) recipients is important because ebv-associated posttransplant lymphoproliferative disease (ptld) can occur after transplantation due to immunosup-pression therapy. methods: to this end we chose real-time pcr using taqman probe. for the standard curve...

Protein purification is an essential procedure in fields such as biochemistry, molecular biology, and biophysics. Acquiring target proteins with high quality and purity is still difficult, although several tag systems have been established for protein purification. Affinity tag systems are excellent because they possess high affinity and specificity for acquiring the target proteins. Neverthele...

Newcastle disease (ND) is one of the most serious illnesses of chickens. Live vaccines are widely used to prevent chicken from the disease all over the world. To access the effective and potentiate ND vaccine, a homogenous subpopulation from LaSota strain was selected following cultivation of the virus on primary chicken embryofibroblast (CEF) cells. Pathogenicity indices and molecular characte...

to carry out the immunological experiments on the serum of multiple sclerosis (ms) patients, based on a correlation between borrelia burgdorferi infection and contracting ms autoimmune disease the outer surface protein d (ospd) of the bacterium was expressed and purified. a clone containing the ospd gene in pet11a expression vector under the control of t7 promoter was transformed to the bacteri...

poplar trees are becoming extremely important as fast growing species due to the increasing need for wood and decreasing productivity of some lands in iran. the present study aimed to find the effect of canopy of poplar clones as one of the most important effective factors on soil properties compared to the distances inter-row trees. five poplar clones and species included: populus euramericana...

background: human cd34 is a transmembrane glycoprotein which is expressed in human hematopoietic stem cells (hscs) and the small- vessel endothelial cells of a variety of tissues. cd34 plays a critical role as a marker for diagnosis and classification of leukemia. anti cd34 antibodies are used for isolation and purification of hscs from bone marrow, peripheral blood and cord blood. objective: t...

Metagenomic analyses can provide extensive information on the structure, composition, and predicted gene functions of diverse environmental microbial assemblages. Each environment presents its own unique challenges to metagenomic investigation and requires a specifically designed approach to accommodate physicochemical and biotic factors unique to each environment that can pose technical hurdle...

Hybridoma clone C3A8 was established as a result of fusion between the lymphocytes of Balb/c mice immunized with the MCF-7 breast carcinoma cell line and Sp2/0-Ag14 myeloma cells. The clone was secreted the monoclonal antibodies (Mab) either in culture supernatant or ascites fluid and still have a contaminants which need to be purify in order to get the desired antibody. The main objective of t...

A method to screen and isolate antigen specific clones from a library of single-chain antibodies expressed on the surface of yeast cells is presented. Two rounds of magnetic bead enrichment before flow cytometric sorting enables one to screen libraries of far greater diversity than can be screened by just flow cytometry. The strength of flow cytometric sorting is the ability to follow the selec...