Ocimum sanctum commonly called (holy basil) an herb containing medicinal, ornamental values, is often used in culinary applications. This research focuses on the improved and efficient protocol for direct regeneration acclimatisation of using nodal segments. Organogenesis multiplication from explants were observed to a maximum Murashige Skoog (MS) medium supplemented with 0.1 mg/L 6-Benzyl amin...

A study of callus induction of Tecomella undulata was conducted using a various concentrations of PGRs namely cytokinins such as BAP and KIN and auxins such as 2, 4-D, NAA and IAA. Internodal explants were aseptically cultured on MS medium supplemented with different concentrations (1.03.0 mg/l) of BAP, KIN, 2, 4-D, IAA and NAA. After two weeks callus was formed at the cut surface of the explan...

Selected 2,6-(disubstituted)purine 2',3'-didehydro-2',3'-dideoxynucleosides and 2',3'-dideoxynucleosides were prepared and evaluated. Treatment of 5'-protected ribonucleosides with phenoxythiocarbonyl chloride and 4-(dimethylamino)pyridine, or under Schotten-Baumann conditions, gave high yields of 2',3'-O-thionocarbonates that underwent Corey-Winter elimination. Treatment of unprotected ribonuc...

The methoxy- and fluoro-derivatives of meta-nitrophenylacetic acid (mNPA) chromophores undergo photodecarboxylation with comparable quantum yields (Φ) to unsubstituted mNPA, but uncage at red-shifted excitation wavelengths. This observation prompted us investigate DPAdeCageOMe (2-[bis(pyridin-2-ylmethyl)amino]-2-(4-methoxy-3-nitrophenyl)acetic acid) DPAdeCageF (2-[bis(pyridin-2-ylmethyl)amino]-...

Establishment of efficient protocol for the induction of somatic embryogenesis and plantlet regeneration from shoot tip explants was achieved in Glycine max (L).Merr. cv.PK472. Highest frequency (95%) of embryogenic callus formation was obtained from shoot tip explants of Glycine max (L).Merr.cv.PK472 on Murashige and Skoog (MS) medium fortified with different concentration of 2,4-Dichloropheno...

a thin-layer chromatography method has been developed and evaluated for the separation and determination of lysine and methonine in a mixture of amino acids obtained from acid or enzyme hydrolysis of protein. the solvent systems were (a) ethanol-ether, (b) phenol-water, (c) ethyl methyl ketone-pyridine-distilled water-glacial acetic acid. procedures in all the three stages were in the same dime...

This research was carried out to investigate the effects of chemical mutagens on producing phenolic compounds in calli obtained from Royal grape variety. Petioles were cultured B5 medium (0.5 mg/L benzyl amino purine and 0.5 indole acetic acid) obtain callus. Calli subcultured same nutrient three times at one month intervals. In fourth subculture, transferred media with composition containing e...

A plant tissue culture protocol from stevia was optimized for the production of planting materials and natural sweetener, rebaudioside A. The highest survivability (88.90% ± 5.55) explants achieved at 15 30 days after initiation (DACI) on Murashige Skoog (MS) media by sterilization with 30% Clorox (5 min) 10% (10 min), respectively. Supplementation MS 0.50 mg/L 2,4-Dichlorophenoxyacetic acid (2...