we find that that expression of claudin 5, a component of endothelial tight junctions, is dramatically down-regulated in Tal1-deficient embryos. Consistent with a defect in tight junction formation, localization of junctional components is disorganized in the Tal1-deficient endocardium. Therefore, we propose that Tal1 transcriptional targets regulate endocardial migration and tube extension thr...

To identify potential targets for aberrant methylation in pancreatic cancer, we analyzed global changes in gene expression profiles of four pancreatic cancer cell lines after treatment with the demethylating agent 5-aza-2'-deoxycytidine (5Aza-dC) and/or the histone deacetylase inhibitor trichostatin A. A substantial number of genes were induced 5-fold or greater by 5Aza-dC alone (631 transcript...

The K+ channel, one of the determinants for neuronal excitability, is genetically heterogeneous, and various K+ channel genes are expressed in the CNS. The therapeutic potential of K+ channel blockers for cognitive enhancement has been discussed, but the contribution each K+ channel gene makes to cognitive function remains obscure. BEC1 (KCNH3) is a member of the K+ channel superfamily that sho...

we find that that expression of claudin 5, a component of endothelial tight junctions, is dramatically down-regulated in Tal1-deficient embryos. Consistent with a defect in tight junction formation, localization of junctional components is disorganized in the Tal1-deficient endocardium. Therefore, we propose that Tal1 transcriptional targets regulate endocardial migration and tube extension thr...

The claudin multigene family is associated with various aberrant physiological and cellular signaling pathways. However, the association of claudins survival prognosis, pathways, diagnostic efficacy in colon cancer remains poorly understood. Methods: Through effective utilization bioinformatics methods, including differential gene expression analysis, set enrichment analysis protein-protein int...

Claudins (Cldns) are transmembrane tight junction (TJ) proteins that paracellularly seal endo- and epithelial barriers by their interactions within the TJs. However, the mechanisms allowing TJ remodeling while maintaining barrier integrity are largely unknown. Cldns and occludin are heterophilically and homophilically cross-over endocytosed into neighboring cells in large, double membrane vesic...

Brain injury after intracranial hemorrhage (ICH) results in significant morbidity and mortality. Blood brain barrier (BBB) disruption is a hallmark of ICH-induced brain injury; however, data mirroring BBB disruption in human ICH are scarce. The aim of this study was to assess the significance of circulating biomarkers in evaluating BBB disruption after ICH. Twenty-two patients with ICH were rec...

The tight junction proteins (TJPs) are major determinants of endothelial cells comprising physiological vascular barriers such as the blood-brain barrier, but little is known about their expression and role in immune cells. In this study we assessed TJP expression in human leukocyte subsets, their induction by immune activation and modulation associated with autoimmune disease states and therap...

Although cardiac fibrosis causes heart failure, its molecular mechanisms remain elusive. In this study, we investigated the mechanisms of cardiac fibrosis and examined the effects of the antifibrotic drug pirfenidone (PFD) on chronic heart failure. To understand the responsible mechanisms, we generated an in vivo pressure-overloaded heart failure model via transverse aortic constriction (TAC) a...