The purpose of this study was to evaluate immunoassay methods for the measurement of serum cardiac creatine kinase isoenzyme (CK-MB) with respect to sensitivity and specificity. The CK-MB electrophoretic assay (Helena Laboratories) was used as the reference. Two principles of immunoassay were included in the evaluation,--immunoinhibition and solid phase separation. The direct immunoinhibition t...

Troponins are recommended as the biomarkers of choice for the detection of myocardial infarction (MI) by the Universal Definition.1 This is well accepted for the diagnosis of type 1 or spontaneous MI, in which any elevation in the setting of ischemia is required but not for the diagnosis of type 4a or MI associated with percutaneous coronary intervention (PCI), in which an isolated 3 the upper ...

BACKGROUND A consensus definition for periprocedural myocardial infarction (MI) in coronary stent trials has not been established. Differences between a historic definition, based on modified World Health Organization (WHO) criteria, and a proposed universal definition have not been compared in a prospective clinical trial. METHODS AND RESULTS We randomly assigned 3687 patients with stable co...

STUDY OBJECTIVES To compare cardiac troponin I (cTnI), cardiac troponin T (cTnT), and creatine kinase MB (CKMB mass) in patients with and without new Q wave on the ECG following coronary artery bypass graft (CABG) surgery. PATIENTS After ethic committee's approval and informed consent, 82 patients, mean age 63+/-10 years, scheduled for CABG were included. INTERVENTIONS Arterial blood sample...

On the other hand, with the emergence of new markers, basically troponins and CKMB mass (“biomarkers of myocardial necrosis”), new evidence began to emerge. For example, many patients who were categorized as having unstable angina have received a diagnosis of acute myocardial infarction without ST segment elevation, because these biomarkers are more sensitive than the enzymes previously used. O...

Results Baseline BNP, TnI and CKMB levels were 105.8 pg/ml, 0.9 ng/ml and 2.34 ng/ml. Although all biomarkers increased significantly within 6 hours of surgery, release kinetics were different. While peak BNP levels occurred in 96% patients by 24-48 hours, for TnI and CKMB, this was observed in only two-thirds. A trend towards fall in all biomarkers was seen by 48 hours; while TnI and CKMB leve...

BACKGROUND The European Society of Cardiology/American College of Cardiology (ESC/ACC) consensus document for definition of myocardial infarction (MI) is predicated on increased cardiac troponin or creatine kinase (CK) MB mass above the 99th percentile reference limit. The purpose of this study was to determine the plasma (heparin) 99th percentile reference limits for the leading in vitro diagn...

PURPOSE Intermittent cold crystalloid cardioplegia by antegrade route to arrest the heart for coronary artery bypass grafting (CABG) is a commonly used technique. The aim of this study is to compare the intermittent antegrade warm blood cardioplegia with cold crystalloid cardioplegia by means of measuring myocardial injury markers CKMB and troponin T. We also compared the results with antegrade...

Introduction: Rhabdomyolysis is a rare and potentially serious adverse drug reaction to antipsychotic medicines. We present a case that developed rhabdomyolysis following the intake of 200 mg olanzapine and 6 gr quetiapine for a suicide attempt. Case Report: A 49-year-old male patient was submitted to the emergency department with complaint of taking an excessive amount of olanzapine (10 mg) an...

PURPOSE This retrospective study sought to determine the benefit of measurement of changes in plasma creatine kinase-myocardial band (CK-MB) levels in elective postoperative high risk surgical patients beyond that obtained from the surface 12 lead ECG. METHODS The charts of 111 patients admitted to the surgical intensive care unit (SICU) of a tertiary level university teaching hospital were r...