Background and Aims: Viroids are smallest known plant pathogens and cause several economically significant diseases. Until recently, viroid detection relied mainly on biological tests and indexing. Today various diagnostic techniques such as nucleic acid hybridization, southern blot and reverse transcription coupled with polymerase chain reaction (RT-PCR) are being used for detection and diag...

viroids are smallest, single-stranded, circular, highly structured plant pathogenic rnas that do not code for any protein. viroids belong to two families, the avsunviroidae and the pospiviroidae. members of the pospiviroidae family adopt a rod-like secondary structure. in this study the most stable secondary structures of citrus viroid variants that reported from fars province were drawn. the m...

The nucleotide sequence and secondary structure of two representative variants from the Group III citrus viroids. CVd-IIIa (297 bases) and CVd-IIIb (294 bases) were determined. The variants are related to the apple scar skin viroid (ASSVd) family. Although smaller in size than any of the ASSVd-related viroids, the central conserved region as well as most of the terminal conserved region of ASSV...

Citrus are affected by many viruses and viroids, some globally widespread restricted to particular countries or areas. In this study, we simulated the use of high throughput sequencing (HTS) bioinformatic analysis small interfering RNAs (siRNA) as a pre-screening method guide bioindexing molecular detection enhance surveillance survey key emerging citrus viruses, such non-European tristeza viru...

background and aims: viroids are smallest known plant pathogens and cause several economically significant diseases. until recently, viroid detection relied mainly on biological tests and indexing. today various diagnostic techniques such as nucleic acid hybridization, southern blot and reverse transcription coupled with polymerase chain reaction (rt-pcr) are being used for detection and diagno...

Abstract Viroids are smallest, single-stranded, circular, highly structured plant pathogenic RNAs that do not code for any protein. Viroids belong to two families, the Avsunviroidae and the Pospiviroidae. Members of the Pospiviroidae family adopt a rod-like secondary structure. In this study the most stable secondary structures of citrus viroid variants that reported from Fars province wer...