Tristeza and Huanglongbing are the most important diseases of citrus caused by Citrus tristeza virus (CTV) and ‘Candidatus Liberibacter’ species, respectively. A reverse transcription duplex polymerase chain reaction (RT-d-PCR) was developed to detect these pathogens simultaneously from infected citrus plants. Consistence of the technique was validated and specificity of the primers was confirm...

The epidemiology of Pierce's disease of grape (Vitis spp.) in California has changed over the past 10 yr due to the introduction of an exotic vector, Homalodisca vitripennis (Germar), the glassy-winged sharpshooter. Although this insect is highly polyphagous, citrus (Citrus spp.) is considered a preferred host and proximity to citrus has been implicated as a significant risk factor in recent ep...

the effects of mandarin scions on peel components and juice quality parameters were investigated in this study. peel flavor components were extracted by using cold-press and eluted by using n-hexane. then all analyzed by gc-fid and gc-ms. total soluble solids, total acids, ph value, ascorbic acid as well as density and ash were determined in juice obtained from mandarin scions. twenty-seven, tw...

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is the primary vector of Candidatus Liberibacter asiaticus (Las) associated with huanglongbing, or citrus greening, the most devastating citrus (Citrus spp.) disease worldwide. Here, we developed a new “excised-leaf assay” that can speed upLas-inoculativity tests on Asian citrus psyllid from the current 3Ð12mo (when usi...

The burrowing nematode, Radopholus similis, attacks agronomic and horticultural crops and many weeds, and is reported to reproduce on more than 250 plant species. Two races of R. similis are recognized. Although one race attacks citrus and the other race does not, they are morphologically similar. At present, the citrus race is found attacking citrus only in Florida, U.S.A., but it is known to ...

The Asian citrus psyllid, Diaphorina citri Kuwayama, vectors Candidatus Liberibacter asiaticus (Las) and Candidatus Liberibacter americanus (Lam), the presumed causal agents of huanglongbing. D. citri generally rely on olfaction and vision for detection of host cues. Plant volatiles from Allium spp. (Alliaceae) are known to repel several arthropod species. We examined the effect of garlic chive...

A range of leaf symptoms, including blotchy mottle, yellowing, and small, upright leaves with a variety of chlorotic patterns resembling those induced by zinc deficiencies, are associated with huanglongbing (HLB, yellow shoot disease), a worldwide destructive citrus disease. HLB is presumably caused by the phloem-limited fastidious prokaryotic α-proteobacterium 'Candidatus Liberibacter spp.' Pr...

The bacterial microbiomes of citrus plants were characterized in response to 'Candidatus Liberibacter asiaticus' (Las)-infection and treatments with ampicillin (Amp) and gentamicin (Gm) by Phylochip-based metagenomics. The results revealed that 7,407 of over 50,000 known Operational Taxonomic Units (OTUs) in 53 phyla were detected in citrus leaf midribs using the PhyloChip™ G3 array, of which f...

Background The citrus industry worldwide has faced major economic losses due to the occurrence of Huanglongbing (HLB), a disease caused by Candidatus Liberibacter spp., Gramnegative bacteria restricted to the phloem of their hosts. Due to the lack of commercial varieties that could resist to these bacteria, genetic transformation comes as an important alternative for improving these varieties a...