The electric potential difference (1 to 15 inv.) between two loci of the slime mold connected with a strand of protoplasm changes rhythmically with the same period (60 to 180 seconds) as that of the back and forth protoplasmic streaming along the strand. Generally some phase difference is observed between them. Periods of the electric potential rhythm show a Gaussian distribution. Amplitudes gi...

The action curve in Chara seems to depend (as in Nitella) on the outward movement of K(+) from the sap. Presumably the increase in permeability in the inner protoplasmic surface and the outward movement of K(+) destroy the concentration gradient of K(+) across the inner protoplasmic surface. Hence the outwardly directed P.D. disappears, causing the rise (spike) of the action curve. The outer pr...

Slime mould Physarum polycephalum builds up sophisticated networks to transport nutrients between distant part of its extended body. The slime mould’s protoplasmic network is optimised for maximum coverage of nutrients yet minimum energy spent on transportation of the intra-cellular material. In laboratory experiments with P. polycephalum we represent Canadian major urban areas with rolled oats...

We have studied semithin frozen sections of developing and adult rat central nervous system (CNS) by indirect immunofluorescence in order to determine the antigenic phenotype of protoplasmic and fibrous astrocytes. Using antibodies against glial fibrillary acidic protein (GFAP) to identify astrocytes, we show that the great majority of fibrous astrocytes in adult optic nerve are labeled by the ...

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We construct electronic oscillator from acellular slime mould Physarum polycephalum. The slime mould oscillator is made of two electrodes connected by a protoplasmic tube of the living slime mould. A protoplasmic tube has an average resistance of 3 MOhm. The tube’s resistance is changing over time due to peristaltic contractile activity of the tube. The resistance of the protoplasmic tube oscil...

It has been shown (Weibull, 1953) that it is possible to isolate protoplasts from Bacillus megaterium by lysozyme treatment of cells suspended in 0.1 to 0.2 M sucrose. When protoplasts are resuspended in phosphate buffer (pH 7), rapid lysis occurs, and the protoplasmic constituents are released. This procedure does not include any mechanical disintegration; in addition, only a small fraction of...