Background Protein production in CHO cell lines is a highly dynamic process, where the yield limiting step can shift among transcription, translation, and secretion under different cell growth stages and culture conditions. In addition, the choice of CHO cell line has been shown to affect target protein quality and quantity. Further advancements in titer and specific productivity require elimin...

The degradation of rat liver tyrosine aminotransferase has been studied after transfection of suitable expression vectors into mammalian cells in culture. A normal rapid rate of degradation (half-life about 6 h) was observed in cells under stable transfection conditions. However, the higher enzyme levels produced during transient transfections or after amplification with methotrexate caused the...

A serum free medium was developed for the production of recombinant antibody against Botulinum A (BoNTA) using dihydrofolate reductase deficient Chinese Hamster Ovary Cells (CHO-DG44) in suspension culture. An initial control basal medium was prepared, which was similar in composition to HAM's F12: IMDM (1:1) supplemented with insulin, transeferrin, selenium and a lipid mixture. The vitamin con...

background: transient gene expression (tge) gained popularity over the last decade as a rapid method for the production of milligram to gram quantities of recombinant proteins for preclinical studies in biophama industry. thereby, the optimization of the tge technique for chinese hamster ovary (cho) as the dominant host for the production of biotherapeutics is of great interest to reach the val...

Background In early development stages of biologicals there is often more than one molecule against a specific target. A careful candidate evaluation is crucial to choose an optimal lead variant for further development. Complex biologicals are typically produced in CHO cells and host cells as well as the process are known to influence important molecule features such as glycan patterns or activ...

For large-scale applications in biotechnology, cultivation of mammalian cells in suspension is an essential prerequisite. Typically, suspension cultures are grown in glass spinner flasks filled to less than 50% of the nominal volume. We propose a superior system for suspension cultures of mammalian cells based on orbital shaker technology. We found that "square-shaped" bottles (square bottles) ...

BACKGROUND Transient Gene Expression (TGE) gained popularity over the last decade as a rapid method for the production of milligram to gram quantities of recombinant proteins for preclinical studies in biophama industry. Thereby, the optimization of the TGE technique for Chinese hamster ovary (CHO) as the dominant host for the production of biotherapeutics is of great interest to reach the valu...

Background Glutamine is an essential component in culture media for most of the mammalian cell lines. It is often used as an alternative source of energy by cells, along with glucose. Glutamine metabolism induces ammonia accumulation in cell culture. Elevated ammonia concentration above 2 mM has been shown to have negative impact on both cell growth and recombinant protein productivity [1-4]. I...