This study investigated the potential of Rhizopus arrhizus UCP 402 for producing chitosan using corn steep liquor and honey as agroindustrial nitrogen and carbon sources. A complete factorial design was used to assess the improved biomass and chitosan production. The results were evaluated using Pareto charts (Statistica 7.0 software). The chitosan obtained was characterized by X-ray diffractio...

A study was conducted to evaluate the effects of chitosan on enteric methane (CH4) and total tract nutrient digestibility of beef heifers consuming either a high-concentrate (HC; 85% concentrate) or a low-concentrate (LC; 36% concentrate) diet. Heifers were provided their respective diets ad libitum along with one of three dietary inclusion rates of chitosan: 1) CTRL=0.0% of chitosan in the die...

Three different Aspergillus Niger MTCC strains (872, 1785 and 2208) were used for the production of chitosan. Multivariate growth mediums in varying incubation periods were analyzed for the production. The produced chitosan was characterized by its physical appearance, moisture content (by means of gravimetric method), percentage of ash and solubility (through the Association of Official Agricu...

The experiments were purposed to investigate the effect of chitosan and yeast extract on Stemona alkaloid production in Stemona sp. culture. Both elicitors enhanced Stemona alkaloid production over the control. Treatment with chitosan at a concentration of 25 mg/L for 1 week resulted in the highest production of Stemona alkaloids. It was found that 1', 2'-didehydrostemofoline and stemofoline pr...

Chitosan multiple addition strategy was developed to improve laccase production from Trametes versicolor cultures. The optimized multiple addition strategy was carried out by two-time addition of 0.1 g L(-1) chitosan to a 2-day-old culture media, with 24-h interval between the treatments. Under these conditions, laccase activity of 644.9 U l(-1) was achieved on the seventh day and laccase produ...

Chitosan is a naturally occurring polysaccharide with antimicrobial properties. This study investigated whether chitosan could induce the LytSR two-component autolytic regulatory system, specifically the cid and lrg operons, found in Streptococcus mutans. A turbidity assay and a murein hydrolase assay were used to observe autolytic activity in chitosan-treated and non-treated cultures of wildty...

Granulocyte macrophage colony stimulating factor, a potent hematopoietic cytokine, has been shown to stimulate production of white blood cells following chemotherapy. Therefore, the granulocyte macrophage colony stimulating factor gene is a potential candidate for the treatment of different pathological conditions. The purpose of this study is to investigate the suitability of chitosan as carri...

The effect of Solid State Fermentation (SSF) on Chitosan production by A. niger was investigated. A. niger BBRC 20004 from Biochemical and Bioenvironmental Research Centre of Sharif University of Technology (Tehran, Iran), was grown on corn residue. Chitosan was extracted from the fungal mycelia using hot alkaline and acid treatment. A. niger was incubated for 12 days on corn residue with moist...

Objective: The purpose of this investigation was to determine the effect of chitosan on osteoclast cells by observing cell viability, bone resorption, and radical oxygen species (ROS) production. Methods: Osteoclast cells were obtained from the primary culture of bone marrow mouse. The osteoclast cells were identified by tartrate-resistant acid phosphatase (TRAP) marker both on the cells and th...

Elicitation with middle-viscous chitosan (30 mg/50 mL) significantly stimulated silymarin synthesis in Silybum marianum hairy root cultures. The root cultures established by infection with Agrobacterium rhizogenes AR15834 showed a potential for production of silymarin. Elicitation with medium molecular weight of chitosan (0, 5, 10, 20, and 30 mg/50 mL) was used in order to improve silymarin pro...