Background: The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. Methods: In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected ...

introduction: heavy ions are nucleus of elements of iron, argon, carbon and neon that all carry positive electrical charges. for these particles to be useful in radiotherapy they need to accelerated to high energy by more than thousand mega volts. also the cosmic environment is considered to be a complicated mixture of highly energetic photons and heavy ions such as iron. therefore, the health ...

Chinese hamster ovary (CHO) cells account for the production of the majority of biopharmaceutical molecules - however, the molecular basis for their versatile properties is not entirely understood yet and the underlying cellular processes need to be characterized in detail. One such process that is supposed to contribute significantly to CHO cell phenotype is methylation of DNA at cytosine resi...

Chinese hamster ovary cells have been in the spotlight for process optimization in recent years, due to being the major, long established cell factory for the production of recombinant proteins. A deep, quantitative understanding of CHO metabolism and mechanisms involved in protein glycosylation has proven to be attainable through the development of high throughput technologies. Here we review ...

Neuroblastoma cells had a much higher rate of glycolysis than the Chinese hamster ovary Kl cells, although the former cell type had a longer doubling time (24 hr) than did the latter (12 hr). DL-Glyceraldehyde inhibited the glycolysis in both cell types to the same extent, but it reduced the growth of neuroblastoma cells more markedly than that of Chinese hamster ovary Kl cells. These data indi...

Using cultured V79 Chinese hamster cells, we found that novobiocin (or 2,4-dinitrophenol) can abrogate, almost completely, the cytotoxicity due to the topoisomerase II inhibitor amsacrine (mAMSA). V79 cells were sensitive to mAMSA killing at all stages in the cell cycle but mainly in S phase followed by late GI phase; however, novo rescued cells of all ages. The properties of two kinds of radia...

Using cultured V79 Chinese hamster cells, we found that novobiocin (or 2,4-dinitrophenol) can abrogate, almost completely, the cytotoxicity due to the topoisomerase II inhibitor amsacrine (mAMSA). V79 cells were sensitive to mAMSA killing at all stages in the cell cycle but mainly in S phase followed by late G1 phase; however, novo rescued cells of all ages. The properties of two kinds of radia...

Chinese hamster ovary (CHO) cell culture systems are the most widely used platform for industrial production of monoclonal antibodies (mAbs). The optimisation manufacturing conditions these high-value products is largely conducted off-line with little or no monitoring mAb quality in-process. Here, we propose DigiGlyc, a hybrid model that predicts critical attribute galactosylation. Having shown...