poultry spermatogonial stem cells (sscs) have the potential to serve as a model for studying the basic biology of ssc and they can also be used for biotechnological purposes. however, the small number of sscs and the presence of the testicular somatic cells with sscs have limited their applications. therefore, this study was undertaken for the first time to investigate the effect of a serum-fre...

Methotrexate (1 muM) abolishes the proliferation of chicken fibroblasts in a culture medium containing defibrinogenated chicken plasma but does not affect proliferation in a medium containing chicken serum.

This study was designed to develop a culture system from the stromal-vascular fraction of chicken adipose tissue that can be used to characterize hormones that promote preadipocyte differentiation. Abdominal adipose tissue was excised from 2 to 4-week-old male broilers (Gallus domesticus) by sterile dissection. The stromal-vascular cell fraction from the adipose tissue was isolated by collagena...

Monoclonal antibodies (MAbs) are invaluable molecules which have several advantages over polyclonal immunoglobulins (Igs) including consistency and higher specificity and hence can be used in biological researches, diagnosis and treatment of diseases. The present study was conducted to produce monoclonal antibody against chicken IgG.TheIgG molecules were purified from chicken serum and used as ...

1. Antisera have been produced in chickens with specific precipitates from Type II pneumococcus horse and rabbit antisera. 2. Specific precipitates from anti-Types I and II pneumococcus horse sera removed the same amount of antibody from the chicken anti-horse specific precipitate serum. Specific precipitates from horse antisera to diphtheria toxin and to crystalline egg albumin removed about o...

Absorption of cobalamin free or bound to chicken serum was assessed in nine patients with pancreatic insufficiency. Simultaneously the in vitro effect of duodenal juice collected from six patients and seven controls was tested on labelled cobalamin complexed to chicken serum or to R salivary binder. Malabsorption of free cobalamin was observed in one of nine patients and in four of nine patient...

The preceding experiments showed that partial or complete inactivation of serum by shaking brought about a marked decrease in the activity of homologous fibroblasts. Generally, serum was not completely inactivated by shaking. In only one instance was the hemo-lytic effect of chicken serum on sheep corpuscles entirely lost after the serum had been shaken for 8 hours. In all other cases, the norm...

The existence of an intermediate in the biosynthesis of serum albumin was suggested by a previous study of the kinetics of appearance of Cl4 in soluble serum albumin in liver slices (1). In the course of investigations on the nature of this intermediate, it has been found that treatment of cytoplasmic particles of chicken liver with deoxycholate or other agents will release non-dialyzable mater...

The four proteins, ovalbumin, conalbumin, lysozyme, and ovomucoid, which comprise the major portion of the proteins of chicken egg white have now been isolated. Quantitative immunochemical investigations of these proteins (l-2) have led to the development of reagents and methods suitable for the study of their distribution in the tissues of the chicken. Such a study has been carried out with eg...

For efficient production of cultured meat, chicken breast muscle cells and leg were proliferated differentiated using medium with various serums, proliferation differentiation ability analyzed. Chicken serum-free (NC), 20% FBS (C), 10% serum (CS) 5% horse (HS) (T1), CS (T2), HS (T3). Using the immunofluorescence staining, Pax7 nuclei stained in to confirm that they satellite cells. As a result ...