The enhanced green fluorescent protein (EGFP) pEGFP-N1-P53 eukaryotic expression vector, which contains the human tumor suppressor p53, was constructed and transfected into chicken fibroblast cells and stage-X blastoderm to analyze the transfection efficiency. The complementary DNA of the human p53 gene was cloned by reverse transcription-polymerase chain reaction from human peripheral blood an...

The propagation of infectious laryngotracheitis virus (ILTV) has been described using primary cell cultures derived from chicken embryo liver and kidney or embryonated eggs, but these cultures use Specific Pathogen Free (SPF) eggs that are time and cost expensive. Since cell line cultures are easier to maintain in laboratory conditions, the growth of ILTV was evaluated in five different cell cu...

RNA-DNA covalent hybrids containing viral RNA have been isolated from nuclear fractions of Rous sarcoma virus-infected chicken embryo fibroblast cells shortly after virus infection. The formation of covalent hybrid structures depends upon a functional reverse transcriptase in vivo, since its appearance in cells is temperature dependent when infected with Rous sarcoma virus mutant LA335, which c...

Abbreviations: MMR: Measles, Mumps and Rubella; WHO: World Health Organization; SSPE: Subacute Sclerosing Panencephalitis; PAHO: Pan American Health Organization; CRS: Congenital Rubella Syndrome; MR: Measles-Rubella; GSK: GlaxoSmithKline; EZ: Edmonston Zagreb; CEF: Chicken Embryo Fibroblast; IBD: Inflammatory Bowel Disease; CDC: Centers for Disease Control and Prevention; SSPE: Subacute Sclero...

objective: the objective of this study was to investigate the pathological alterations of methionine for chicken embryo.in recent years, amino acids are used in veterinary medicine for many purposes. they injected into hatching eggs to increase chick body weight and performance at hatch, but the adverse effects of amino acids have always been a major concern. there is scant information availabl...

Previous studies showed that the retina produces factors that promote the differentiation of lens fiber cells, and identified members of the fibroblast growth factor (FGF) and insulin-like growth factor (IGF) families as potential fiber cell differentiation factors. A possible role for the bone morphogenetic proteins (BMPs) is suggested by the presence of BMP receptors in chicken embryo lenses....

An outbreak of goatpox was attended in district Durg and surrounding regions of Chhattisgarh state. Dried skin scabs were collected from 250 goats of different age groups showing clinical signs suggestive of pox. The prevalence rate was studied based on severity of clinical signs followed by confirmation with agar gel immuno diffusion (AGID) test. Positive scabs were further processed for virus...

Silver nanoparticles (AgNPs) are broadly designed for various application. In the current research silver have been examined their poisonous outcome on human lymphocyte and chicken embryo fibroblast cell line. AgNPs were synthesized by a simple easy method from AgNO3 solution with glucose exposed to microwave radiation. The typical size of 30 nm spherical shape. cells which cultured diverse con...

nitroimidazole compounds have been traditionally used in the veterinary and human medicine, but their uses are sometimes associated with some side effects. toxicopathological effects of nitroimidazoles have always been a major concern. there is scant information available about the pathological alterations of nitroimidazole compounds in the embryo. the objective of this study was to determine t...