A previous study of random mutations, mostly introduced by error-prone PCR (EPPCR) or DNA shuffling (DS), demonstrated that those closer to the enzyme active site were more effective than distant ones at improving enzyme activity, substrate specificity or enantioselectivity. Since then, many studies have taken advantage of this observation by targeting site-directed saturation mutagenesis (SDSM...

Measuring substrate and/or product concentration can create a major bottleneck for synthetic and biosynthetic processes. Here we report the development and substrate screening of a whole cell biosensor to detect biomass-derived aromatic chemical building blocks, supporting the use of sustainable feedstocks in the bulk and fine chemical industries.

Recently, Sevag & Colton (1959) published a chemical test for detecting the precise day of ovulation. We have performed this test in 112 menstrual cycles of sixty women. Out of 112 menstrual cycles studied, in fifty-six menstrual cycles (50%) the chemical patterns were atypical. We do not substantiate the claim of Sevag & Colton that the ovulation invariably occurs between 10th and 14th days of...

purpose: to assess whether bromocriptine-rebound method (brm) can improve pregnancy   outcomes compared to long protocol after intracytoplasmic sperm injection cycles (icsis).   materials and methods: a total of 114 women underwent icsi. pregnancy outcomes and   hormonal data were compared between two groups, i.e. long protocol and brm. ovulatory   women with normal serum prolactin levels were ...

background: frozen- thawed embryo transfer is an essential part of art treatment and outcomes of this procedure are associated with several clinical factors. several studies have showed an increase level of ivf outcomes in women with sufficient vitamin d. objective: whether treatment of vitamin d insufficiency can improve pregnancy rates in frozen-thawed embryo transfer cycles. materials and me...

abstract soil drying and rewetting (drw) cycles are considered as environmental stress that would affect the physical, chemical and biological properties of soil and nutrient cycles. the study of soil drying and rewetting effects on plant nutrition and soil fertility is very important and crucial specially their environmental effects. in this study, the effects of 1, 2, 3, 4 and 5 drying and re...

In order to assess mineralogical composition influence on durability and strength of rocks, four samples of hornfelsic rocks were selected from southern and western parts of the city of Hamedan, west of Iran. These rock samples were subjected to mineralogical, physical and mechanical tests in the laboratory. Also, they were evaluated in 15 cycles of slake-durability testing in different pH of s...