Background: Formation of secondary structure such as DNA hairpins or loops may influence molecular genetics methods and PCR based approaches necessary for genetic engineering, in addition to gene regulation. Materials and Methods: A polymerase chain reaction with splice overlap extension (SOE-PCR) was used to create fully synthetic 1F5 chimeric anti-CD20 heavy- and light-chain genes. The chi...

Objective(s): We previously conducted an in silico research on the interactions between the ribosome display-selected single chain variable fragment (scFv) and factor H binding protein (fHbp) of Neisseria meningitidis. We found that heavy chain variable (VH) fragment of this scFv had considerable affinity to fHbp. These results led us to evaluate the ability of this sm...

VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was clonedin a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leadersequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Theconstruct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of...

Background:Epidermal growth factor receptor (EGFR) overexpression is a characteristic of several malignancies and could be considered as an excellent target for designing specific inhibitors such as anti-EGFR monoclonal antibodies for cancer therapy. Drawbacks exerted by large sizes of full-length antibodies have lead to the development of single chain antibodies, which benefit from having smal...

background: formation of secondary structure such as dna hairpins or loops may influence molecular genetics methods and pcr based approaches necessary for genetic engineering, in addition to gene regulation. materials and methods: a polymerase chain reaction with splice overlap extension (soe-pcr) was used to create fully synthetic 1f5 chimeric anti-cd20 heavy- and light-chain genes. the chimer...

vp2 gene coding region of a vaccinal strain (d78) of infectious bursal disease virus (ibdv) was clonedin a eukaryotic expression vector, psec tag2a. the gene was placed downstream of ig κ chain leadersequence, under the control of human cytomegalovirus (hcmv) immediate early enhancer and promoter. theconstruct psec tag2a-vp2 was transfected in cos-7 cell line and the expression and secretion of...

Antibody modelling by side chain placement on an homologous backbone conformation is a combinatorially hard problem. This can be made tractable by using a knowledge-based approach in which a heuristic cost function is used to perform a structural search on a database of known structures in order to nd re-usable clusters of side chains. The procedure described yields a plausible structure which ...

BACKGROUND The connection of the variable part of the heavy chain (VH) and and the variable part of the light chain (VL) by a peptide linker to form a consecutive polypeptide chain (single chain antibody, scFv) was a breakthrough for the functional production of antibody fragments in Escherichia coli. Being double the size of fragment variable (Fv) fragments and requiring assembly of two indepe...

With the aim of the production of human factor VIII antigen and its corresponding antibody an epitope coding fragment of the light-chain of hFVIII, fused to a His6-tag, was isolated and over-expressed in Escherichia coli. The over-expressed hFVIII-epitope containing peptide was confirmed by its reaction with a rabbit serum directed against native hFVIII as well as antiHis6-tag antibody. An expr...