objective(s): valproic acid (vpa) has proven to be as one of the most promising useful drug with anticancer properties.in this study, we investigate the vpa effects on e-cadherin expression in hela, tc1, mkn45, and hct116 cell lines.  this study assesses the effects of vpa on human papillomavirus e7 expression in hpv positive cell lines. materials and methods: cell lines were treated by2 mmol/l...

Previous studies show that human myeloma-derived cell lines specifically adhere to fibronectin (FN) through very late antigen-4 (VLA-4; alpha 4 beta 1 integrin complex) and RGD-peptide mechanisms, which may contribute to the localization of tumor cells in bone marrow (BM). In these studies, we characterized the adhesion of myeloma-derived cell lines to both normal and myeloma BM stromal cells (...

Thrombospondin is a 420-kD platelet alpha-granule glycoprotein that binds specifically to heparin. We examined adhesion to thrombospondin of CHO K1 cells and three mutant CHO lines with varying deficiencies in glycosaminoglycan (GAG) synthesis. In an experiment in which the parent line (K1) had 78% adherence to thrombospondin adsorbed to tissue culture plastic, CHO S745 cells, with less than 6%...

obtaining a continuous source of normal cells or dna from a single individual has always been a rate limiting step in biomedical research. availability of lymphoblastoid cell lines (lcls) as a surrogate for isolated or cryopreserved peripheral blood lymphocytes has substantially accelerated the process of biological investigations. lcls can be established by in vitro infection of resting b cell...

the optimization of cell culture conditions for growth and productivity of recombinant chinese hamster ovary (cho) cells is a critical step in biopharmaceutical manufacturing. in the present study, the effects of the timing and amount of peptone feeding of a recombinant cho cell line grown in a basal medium in serum-free suspension culture were determined for eight peptones of different origin ...

Microsporidia are obligate intracellular opportunistic protists that infect a wide variety of animals, including humans, via environmentally resistant spores. Infection requires that spores be in close proximity to host cells so that the hollow polar tube can pierce the cell membrane and inject the spore contents into the cell cytoplasm. Like other eukaryotic microbes, microsporidia may use spe...

Objective(s): Valproic acid (VPA) has proven to be as one of the most promising useful drug with anticancer properties.In this study, we investigate the VPA effects on E-cadherin expression in HeLa, TC1, MKN45, and HCT116 cell lines.  This study assesses the effects of VPA on human papillomavirus E7 expression in HPV positive cell lines. Materials and Methods: Cell lines were treated by2 mmol/...

conclusions collectively, the v. pumilum extracts showed remarkable cytotoxic activity and merit further investigation as anticancer agents. results the ch2cl2 fraction of v. pumilum showed a potent dose-related inhibitory effect on cell viability. the ch2cl2 fraction showed ic50 values of 8.3 and 12.5 µg/ml in the k562 and hl-60 cell lines, respectively. a sub-g1 peak in the flow cytometry his...

abstract background: the aims of this study were to assay the activity of adenosine deaminase (ada) in estrogen receptor positive (mcf-7) and negative (mda-mb468) breast cancer cell lines. methods: mda-mb468 and mcf-7 breast cancer cell lines were cultured in complete medium, striped serum with and without 0.0 1~-lm diethylstilbestrol (des), complete medium in the presence and absence of 111m t...

The present study was designed to investigate the in-vitro morphological assessment of apoptotic effect caused by nimbolide on the selected cancer cell lines (DU-145, PC-3, A-549) and normal cell lines (NIH3T3, CCD-18Co). The cells were grown in 6 well tissue culture plates after treatment with different concentrations of nimbolide and untreated control cells. Apoptotic and necrotic ce...