Objective(s):Brucellosis is a well-known domestic animal infectious disease, which is caused by Brucella bacterium. GroEL antigen increases Brucella survival and is one of the major antigens that stimulates the immune system. Hence, the objective of the present study was cloning and bioinformatics analysis of GroEL gene. Materials and Methods: The full-length open reading frame of this gene was...

infectious bursal disease (ibd), a highly contagious and devastating disease in young chicken, is causedby infectious bursal disease virus (ibdv). to improve the immunogenicity of recombinant ibdv subunitvaccine, an attempt was made to find a new way to prepare ibd vaccine containing glycosylated mvp2antigen. firstly, ibdv mvp2 gene (with a nucleic acid sequence encoding b cell epitope of ibdv(...

human cytomegalovirus is one of the most common pathogenic viruses all over the world. in congenital infection leads to neurologic severe disorders and even death of fetus and in individuals with immunosuppression may also cause severe clinical symptoms. multiple evidence indicate that among several strategies, epitope-based vaccine (evs) that can induce both humoral and cellular immunity respo...

Brucellosis, caused by the genus Brucella bacterium, is a well-known infection among domestic animals. Considering the serious economic and medical consequences of this infection, various preventive efforts have been made through using recombinant vaccines, based on outer membrane protein (OMP) antigens of Brucella species. The objective of the present study was to clone, analyze the sequence, ...

Infectious bursal disease (IBD), a highly contagious and devastating disease in young chicken, is causedby infectious bursal disease virus (IBDV). To improve the immunogenicity of recombinant IBDV subunitvaccine, an attempt was made to find a new way to prepare IBD vaccine containing glycosylated mVP2antigen. Firstly, IBDV mVP2 gene (with a nucleic acid sequence encoding B cell epitope of IBDV(...

conclusions hcv gpe2 is an ideal target for hcv vaccine. prediction of epitope immunogenicity and characterization on the basis of peptide sequences will be significantly helpful for development of a heterologous vaccine against hcv variants. results many b-cell and t-cell epitopes predicted in gpe2 were found conserved among hcv 3a genotypes whereas few were conserved in other genotypes antici...

CD8+ T cells are thought to play an important role in protective immunity to tuberculosis. The major histocompatibility complex class I subtype HLA-A*0201 is one of the most prevalent class I alleles, with a frequency of over 30% in most populations. HLA-A*0201 transgenic, H-2Db/mouse beta2-microglobulin double-knockout mice (HHD) which express human HLA-A*0201 but no mouse class I, was shown t...

• Increased T-cell responses after targeting antigen to antigen-presenting cells. Antibody:T-cell epitope fusion mode affects the strength of response. integration supports induction CD4 and CD8 responses. Targeted delivery cells (APCs) enhances presentation thus, is a potent strategy for making more efficacious vaccines. This can be achieved by use antibodies with specificity endocytic surface...

cd8+ t cells are thought to play an important role in protective immunity to tuberculosis. the major histocompatibility complex class i subtype hla-a*0201 is one of the most prevalent class i alleles, with a frequency of over 30% in most populations. hla-a*0201 transgenic, h-2db/mouse beta2-microglobulin double-knockout mice (hhd) which express human hla-a*0201 but no mouse class i, was shown t...

Brucellosis is a well-known infection in domestic animals which caused by Brucella bacterium. Due toserious economic and medical consequences of this disease, various efforts have been made to prevent theinfection through the use of recombinant vaccines based on Brucella outer membrane protein (OMP)antigens. The objectives of the present study were cloning, sequencing and epitope prediction of ...