نتایج جستجو برای: cdna sequence
تعداد نتایج: 431646 فیلتر نتایج به سال:
Purpose: The aim of this study was cloning the Gba enzyme in pUCBM21 plasmid, and making frame mutation on it and sequencing it. Materials and methods: mRNA was extracted from mouse spleen and glucocerebrosidase cDNA was synthesized and amplified by PCR with specific primers. cDNA was cloned in pUCBM21 and analyzed by restriction enzymes. A fragment of its sequence was deleted using MscI restr...
background: because of the strong immunologic responses of surface protein tasp in theileria annulata infected host, we tried to characterize this protein in a t. annulata isolate from iran. methods: the rna prepared from t. annulata infected cells was used to produce smart-ds-cdna. the double strand cdna was then amplified with primers derived from tasp mrna sequences. the pcr product was cl...
aspartic proteases are a relatively small group of enzymes which express in various nematodes including onchocerca volvulus. an estimation of the gene copy number corresponding to the ov7a clone, which contains a cdna insert encoding approximately two-thirds of the entire coding sequence of aspartic protease of o. volvulus, was made by slot blot analysis in a closely related species o. gibsonig...
Background: The cultivation of saffron is expanding through the southeast of Iran, and allergy to saffron pollen occurs in workers involved in processing this plant. We aimed to clone, sequence and express a major allergen involved in saffron pollen allergy, and to compare the recombinant with the natural allergen. Methods: The N-terminal amino acid sequence of Cro s 1, an allergen from saff...
Apolipoprotein A-I is the most abundant protein in Cyprinus carpio plasma that plays an important role in lipid transport and protection of the skin by means of its antimicrobial activity. A 527 bp cDNA fragment encoding C terminus part of apoA-I from the skin mucosa of common carp was isolated using RT-PCR. After GenBank database searching, a partial sequence containing a coding sequence (CDS)...
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