An improved pH-stat-technique for the determination of carbonic anhydrase is presented. As compared with common techniques the main advantages are: 1. The hydration reaction of CO2 catalyzed by carbonic anhydrase can be determined at a constant and selected pH-value, 2. the substrate concentration can be kept constant, 3. the reaction volume amounts to only 2 ml, and 4. the reaction can be meas...

Article history: Received 20 August 2014 Received in revised form 19 September 2014 Accepted 23 October 2014 Available online 16 November 2014

It's the water that matters. Pairs of benzo- and perfluorobenzoarylsulfonamide ligands bind to human carbonic anhydrase with a conserved binding geometry, an enthalpy-driven binding, and indistinguishable binding affinities (see picture). These data support the pervasive theory that the lock-and-key model disregards an important component of binding: the water, which fills the binding pocket of...

A structural study of the adduct which 2-benzylsulfinylbenzoic acid forms with human carbonic anhydrase II is reported, showing a binding mode completely different from any other class of carbonic anhydrase inhibitors investigated so far; this carboxylate binds in a pocket situated out of the enzyme active site.

Most measurements of blood carbonic anhydrase activity recorded in the literature have been made by the method of Meldrum and Roughton (1) or some modification of it. These procedures are carried out in a cold water bath at 10” or below. This temperature is disadvantageous technically, and, moreover, affords no direct information as to the activity of the enzyme at mammalian body temperature; i...

Red cell carbonic anhydrase activity, 2.3 DPG concentration, and activities of three key enzymes controlling DPG metabolism (PK, PFK and DPGM) were measured in normoxic and hypoxic (incubation in 13.5% O2) chick embryos. In normoxia 2.3 DPG concentration and carbonic anhydrase activity begin to increase by the third week of incubation. Hypoxia induces a rise of 2.3 DPG concentration and carboni...

The presence of a carbon-concentrating mechanism in the symbiotic dinoflagellate Symbiodinium sp. was investigated. Its existence was postulated to explain how these algae fix inorganic carbon (C(i)) efficiently despite the presence of a form II Rubisco. When the dinoflagellates were isolated from their host, the giant clam (Tridacna gigas), CO(2) uptake was found to support the majority of net...