this study was conducted to investigate the effect of physical and chemical processing on crude protein fractions of two varieties of canola (okapi and modena) and safflower (62 and zarfan) seed using cornel net carbohydrate and protein system and on protein subunits using sodium dodecyl sulfate polyacrylamide gel electrophoresis. formaldehyde was used as chemical treatment, while toasting and ...

Rat and human colonic mucin glycoproteins bind to the Gal/GalNAc adherence lectin on the surface of Entamoeba histolytica in vitro, thus inhibiting the organism from adhering to and lysing the target cells. Human colonic mucin glycoproteins were isolated by Sepharose 4B gel filtration chromatography, they were proteolytically degraded with trypsin, pronase, and papain, and the glycoprotein frac...

The isolation of the principal glycoprotein from human gastric aspirates and the determination of its carbohydrate and amino acid composition is described.Ninety-nine individual gastric aspirates were investigated. Eighty-two were eluted on Bio-gel P150 and the carbohydrate and amino acid composition of each non-retarded fraction was determined. Fifteen of these non-retarded fractions were chro...

The current biorefineries are focused on the comprehensive fractionation of biomass components into separate lignin and carbohydrate fractions for production materials, platform chemicals biofuel.

BACKGROUND AND AIMS Developing a conceptual and functional framework for simulating annual long-term carbohydrate storage and mobilization in trees has been a weak point for virtually all tree models. This paper provides a novel approach for solving this problem using empirical field data and details of structural components of simulated trees to estimate the total carbohydrate stored over a do...

Ferritin isolated from porcine spleen could routinely be separated in two fractions on nondenaturating gradient gels. Both fractions could be isolated with a purity of 96% when applied to two serially linked columns, each 200 cm in length, packed respectively with Sepharose 4B and Sepharose 6B. Both fractions were similar as judged by electron microscopy. Assessed biochemically fractions were e...

Anorexia, nausea, and vomiting are common symptoms of uremic intoxication. Fractions in the middle molecule weight range, isolated from normal urine and uremic plasma ultrafiltrate, inhibit ingestive behavior in the rat. To investigate their site of action and specificity, male rats were injected intraperitoneally, intravenously, or intracerebroventricularly with concentrated fractions of uremi...

Sulfated glycoproteins were purified from tryptic digests of hog stomach mucosa. The sulfated glycoprotein fractions were isolated by ethanol and cetylpyridinium chloride precipitation followed by free-flow electrophoresis. The separation of the sulfated glycoproteins was performed by elution from DEAE-Sephadex A-50 with NaCl. After gel filtration of the major fractions on Bio-Gel A-5Om, final ...

Mycoplasma hyorhinis organisms were fractionated into lipid, carbohydrate, and protein fractions by chemical and physiochemical procedures. When all fractions were tested for complement fixation and inhibition of growth inhibition activity, only the protein fractions possessed activity. Thus, it appears that the major antigenic component of M. hyorhinis is a protein.