pCAR1 endows carbazole-degrading ability on its host. Three different host Pseudomonas (P. fluorescens Pf0-1, P. resinovorans CA10dm4, and P. chlororaphis IAM1511) were prepared to compare their behaviors in artificial microcosms (sterile soil and water supplemented with carbazole) with previous results for P. putida SM1443 (Shintani et al., Appl. Microbiol. Biotechnol., 80, 485-497 (2008)). We...

We determined the complete 254,797-bp nucleotide sequence of the plasmid pCAR3, a carbazole-degradative plasmid from Sphingomonas sp. strain KA1. A region of about 65 kb involved in replication and conjugative transfer showed similarity to a region of plasmid pNL1 isolated from the aromatic-degrading Novosphingobium aromaticivorans strain F199. The presence of many insertion sequences, transpos...

Using vo1tammetric technique with a rotating disk electrode, the anodic oxidation of carbazole in aqueous H2S04 ethanol mixtures was shown to proceed under diffusion and electron transfer control conditions. Electro-oxidation involves one electron transfer; electrode passivation occurs due to the formation of a poorly soluble product. By means of the SCF MO LCAO INDO, AMI-UHF and PM3-UHF method...

In the title mol-ecule, C(26)H(25)Br(3)N(2), a dihedral angle of 6.15 (10)° is present between the carbazole and benzene ring systems with an E conformation about the C=C bond [1.335 (4) Å]. The butyl group is almost perpendicular to the carbazole plane [C-N-C-C torsion angle = -98.7 (3)°]. In the crystal, supra-molecular double chains along [-7,18,-16] are formed via C-H⋯Br and π-π inter-actio...

Nitrogen heterocyclic compounds, especially carbazole, quinolone, and pyridine are common types of environmental pollutants. Carbazole has a toxic influence on living organisms, and the knowledge of its persistence and bioconversion in ecosystems is still not complete. There is an increasing interest in detoxification of hazardous xenobiotics by microorganisms. In this study, the ability of thr...

In the title compound, C(19)H(19)FN(2)O(2)S, the hydrogenated six-membered ring of the carbazole unit adopts a half-chair conformation and the plane of the fluoro-phenyl ring forms a dihedral angle of 41.5 (1)° with respect to the carbazole mean plane. The crystal structure is segregated into layers containing the carbazole units and fluoro-phenyl rings in alternate (200) planes. The carbazole ...

In the title compound, C(14)H(15)NO(2), the hydrogenated six-membered ring of the carbazole unit adopts a half-chair conformation and the dioxolane ring points to one side of the carbazole plane. Neighbouring mol-ecules form edge-to-face inter-actions in which the NH group is directed towards an adjacent carbazole unit, with a shortest H⋯C contact of 2.72 Å. These inter-actions arrange the mol-...

In the title compound, C(18)H(21)NO(4), the hydrogenated six-membered ring of the carbazole unit adopts a half-chair conformation. The dioxolane ring and ethyl-acetate substituent point to opposite sides of the carbazole plane. The ethyl-acetate substituent adopts an essentially fully extended conformation, and its mean plane forms a dihedral angle of 83.8 (1)° with respect to the carbazole mea...

Carbazole 1,9a-dioxygenase (CARDO), a Rieske nonheme iron oxygenase (RO), is a three-component system composed of a terminal oxygenase (Oxy), ferredoxin, and a ferredoxin reductase. Oxy has angular dioxygenation activity against carbazole. Previously, site-directed mutagenesis of the Oxy-encoding gene from Janthinobacterium sp. strain J3 generated the I262V, F275W, Q282N, and Q282Y Oxy derivati...

In the present work we report a gram negative bacterial isolate, from soil of a dye industry, with promising biorefining and bioremediation potential. This isolate (GBS.5) could utilize carbazole (nitrogen containing polycyclic aromatic hydrocarbon) as the sole source of nitrogen and carbon and utilize almost 98% of 3mM carbazole in 100 hours. The specific activity of our GBS.5 isolate for carb...