A selection of 58 specimens with a monoclonal component identified by immunoelectrophoresis and/or immunofixation was analyzed with the immunosubtraction procedure on the Paragon 2000 capillary electrophoresis system. The capillary system detected 93% of the paraproteins and, using immunosubtraction, correctly identified 91% of the paraproteins. Paraproteins that were detected by immunofixation...

An equation analogous to the Van Deemter equation in chromatography is developed to account for peak broadening in capillary zone electrophoresis (CZE). This equation applies to conditions where the peaks are symmetrical (sample zone concentration much less than background electrolyte concentration). To identify and to quantitate the effects of different contributors to the peak width in CZE, i...

(29) Pszenny, A. A. P.: Harvey, G. R.; Brown, C. J.; Lang, R. F.; Keene, W. (21) Hendbook of ChemistryandPhysics, 56th ed.; CRC Press: Cleveland, OH, 1975. (22) Van Wijk, R. J. Chromatogr. Sci. 1970, 8 , 418-420. (23) Bertsch, W.: Chang, R. C.; Zlatkis, A. J. Chromatogr. Sei. 1974. 72, 175-182. (24) Butler, L. D.; Burke, M. F. J. Chromatogr. Sci. 1976, 74, 117-122. (25) Lang, R. F. Anal. Chem. ...

Applying isotachophoresis (ITE’) superimposed on capillary zone electrophoresis (CZE), which will be denoted the ITPlCZE mode, components migrate in an ITP way on top of a background electrolyte. In such an ITPKZE system the leading electrolyte consists of a mixture of an ionic species L, with a high mobility (the leading ion of the ITP system), an ionic species L, with low mobility (the co-ion...