PURPOSE To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials...

Pretreatment of recipients with the monoclonal antibody (MoAb) S5 facilitates engraftment of bone marrow from mismatched, unrelated donors in the canine transplantation model. In the direct comparisons reported here, the S5 glycoprotein (gp) was found to have structural homology to CD44 that in humans has been implicated in adhesive interactions of one type of effector cell, the lymphocyte. The...

Multiple myeloma (MM) or plasma cell myeloma is a neoplastic proliferation of plasma cells that primarily involves the bone marrow but may originate from extramedullary sites [1-4]. Although it is uncommon in veterinary medicine, it has been reported in several species, in‐ cluding cats, dogs and, horses [1,3,5-10]. The frequency of MM in cats is slightly <1% of all malignant neoplasms. Canine ...

Mesenchymal stem cells (MSCs) - usually obtained from bone marrow - often require expansion culture. Our protocol uses clinical grade urokinase to degrade clots in the bone marrow and release MSCs for further use. This protocol provides a rapid and inexpensive alternative to bone marrow resampling. Bone marrow is a major source of MSCs, which are interesting for tissue engineering and autologou...

Background: Bone marrow is the site of many malignant disorders and it is one of the common places for solid tumors to metastasize. Examination of the bone marrow aspirate and biopsy is a routine procedure performed for assessment of various conditions such as cytopenias, hematologic neoplasms, nonmalignant disorders and metastatic neoplasms. Methods: The patients were referred to the Hema...

Canine monocytic ehrlichiosis (Ehrlichia canis infection) is a serious tick-transmitted disease of dogs that was considered exotic to Australia until recently. The first reported across northern and central in 2020, with significant canine morbidity mortalities observed at indigenous communities where dog numbers are high, ticks superabundant, tick prevention scant. date location the incursion ...

OBJECTIVES Severe intervertebral disc herniation causes complete paraplegia and loss of pain sensation in canines. The prognosis is poor, even when decompression surgery is performed immediately after onset. Studies suggest that bone marrow-derived mononuclear cells will regenerate the injured spinal cord and restore neurologic function. This study was conducted to assess the clinical efficacy ...

Optimization of retroviral gene transfer into hematopoietic cells of the dog will facilitate gene therapy of canine X-linked severe combined immunodeficiency (XSCID) and in turn advance similar efforts to treat human XSCID. Both canine and human XSCID are caused by defects in the common gamma chain, gammac, of receptors for interleukin-2 and other cytokines. In this study, normal dogs were give...

A 13-year-old neutered male Maltese dog presented to our hospital with lethargy and anorexia. Laboratory abnormalities included severe non-regenerative anemia (hematocrit, 12.9%; reticulocyte count 12.8 K/μL). The cytology of bone marrow revealed erythroid hypercellularity mild myelofibrosis. Therefore, late-stage precursor-targeted immune-mediated (PIMA) was diagnosed. Multimodal treatment inc...