نتایج جستجو برای: candidatus phytoplasma pyri
تعداد نتایج: 4377 فیلتر نتایج به سال:
X-disease is one of the most serious diseases known in peach (Prunus persica). Based on RFLP analysis of 16S rRNA gene sequences, peach X-disease phytoplasma strains from eastern and western United States and eastern Canada were classified in 16S rRNA gene RFLP group 16SrIII, subgroup A. Phylogenetic analyses of 16S rRNA gene sequences revealed that the X-disease phytoplasma strains formed a di...
In this paper the validation and implementation of a Real-time PCR protocol based on ribosomal protein genes has been carried out for sensitive and specific quantification of 'Candidatus (Ca.) Phytoplasma mali' (apple proliferation phytoplasma, APP) in insects. The method combines the use of EvaGreen(®) dye as chemistry detection system and the specific primer pair rpAP15f-mod/rpAP15r3, which a...
The genetic diversity of three temperate fruit tree phytoplasmas 'Candidatus Phytoplasma prunorum', 'Ca. P. mali' and 'Ca. P. pyri' has been established by multilocus sequence analysis. Among the four genetic loci used, the genes imp and aceF distinguished 30 and 24 genotypes, respectively, and showed the highest variability. Percentage of substitution for imp ranged from 50 to 68 % according t...
a survey was carried out in citrus nurseries in mazandaran and kerman provinces as the major citrus growing regions of iran. different varieties of orange and tangerine with abnormal symptoms were examined for the occurrence of candidatus liberobacter asiaticus, spiroplasma citri and phytoplasma as the most important vascular limited pathogens in citrus. by visual inspection and molecular exami...
Article Information Brinjal is an important vegetable crop grown worldwide. A field survey conducted during 2012 in Kerala state of South India, the little leaf disease affected brinjal plant was observed in one of the home gardens located at Nemmara (Palakkad district). The diseased and healthy samples were collected; genomic DNA was isolated and 16SrRNA gene amplified by performing nested PCR...
The discovery in 1967, of a new group of plant pathogens related to bacteria led to the finding of pleomorphic, wall-less prokaryotes in the phloem of many plant species affected by yellows-type diseases. Following the application of molecular technologies the phylogeny of these prokaryotes was resolved and led to the new trivial name of “phytoplasma” and to the designation of a new taxon named...
A phytoplasma was detected in golden beard grass (Chrysopogon acicalatus) displaying white leaf symptoms near vegetative fields at the Tatkone region in Myanmar, 2011. Based on restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes, including the 16S-23S spacer region and part of the 23S rRNA gene, the phytoplasma was identified as a member of the Bermuda grass white ...
A molecular tool, focused on the mitochondrial Control Region (CR), was developed to discriminate the two hawthorn-feeding psyllid species, Cacopsylla melanoneura (Förster) and C. affinis (Löw), and to estimate their frequencies in mixed populations. The test was carried out in paired and single-tube amplifications and validated analysing 52 male specimens previously determined by morphological...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید