Cells with sphere forming capacity, spheroid cells, are present in the malignant ascites of patients with epithelial ovarian cancer (EOC) and represent a significant impediment to efficacious treatment due to their putative role in progression, metastasis and chemotherapy resistance. The exact mechanisms that underlie EOC metastasis and drug resistance are not clear. Understanding the biology o...

The cancer stem cell theory hypothesizes that cancer stem cells (CSCs), which possess self-renewal and other stem cell properties, are regarded as the cause of tumor formation, recurrence and metastasis. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs. In this study, we enrich...

Colorectal cancer (CC) is an important human malignancy with high related death worldwide. The chemotherapy using doxorubicin hydrochloride one of the most common therapeutic methods. However, drug resistance lowers treatment efficacy in CC patients. combination therapies seem to be more promising by taking advantage synergistic effects. present study aimed evaluate a new strategy enhance antic...

The growth of cancer cells as multicellular spheroids has frequently been reported to mimic the in vivo tumour architecture and physiology and has been utilized to study antitumour drugs. In order to determine the distinctive characteristics of the spheroid-derived cells compared to the corresponding monolayer-derived cells, we enriched multicellular spheroid-forming subpopulations of cells fro...

background: gastric cancer (gc) is widely associated with chronic inflammation. the pro inflammatory microenvironment provides conditions that disrupt stem/progenitor cell proliferation and differentiation. the signal transducer and activator of transcription- 3 (stat3) signaling pathway is involved in inflammation and also contributes to the maintenance of embryonic stem cell (escs) pluripoten...

Introduction: Breast cancer is the most common metastatic malignancy and the second leading cause of death in women. Recently, extremely low-frequency electromagnetic fields (ELF-EMFs) seem to modulate the rate of proliferation and enhance apoptosis and are considered as an emerging approach to cancer therapy. Despite recent success in the electromagnetic fields, the results are still neither d...

background : biomarkers accepted for clinical use in breast cancer have low sensitivity and specificity. thus, there is a need for new markers to assist in the diagnosis, prognosis and follow-up of breast cancer patients. this study aims to investigate the diagnostic, prognostic and follow-up role of serum bcl-2, bax and p53 proteins in breast cancer patients in comparison with those of serum c...

This paper presents a multicellular spheroid chip capable of forming and extracting three-dimensional (3D) spheroids using removable cell trapping barriers. Compared to the conventional macro-scale spheroid formation methods, including spinning, hanging-drop, and liquid-overlay methods, the recent micro-scale spheroid chips have the advantage of forming smaller spheroids with better uniformity....

The cancer stem cell (CSC) theory hypothesizes that CSCs are the cause of tumor formation, recurrence and metastasis. Key to the study of CSCs is their isolation and identification. The present study investigated whether spheroid body-forming cells in the human gastric cancer (GC) MKN-45 cell line are enriched for CSC properties, and also assessed the expression of the candidate CSC markers, oc...