Previously, we demonstrated that epinephrine induced the expression of interleukin (IL)-6 mRNA via beta-adrenoceptors in cultured human osteoblastic cells. IL-6 is well known to modulate bone metabolism by regulating the development and function of osteoclasts and osteoblasts. Recently, restraint stress and intracerebroventricular injection of lipopolysaccharide (LPS) have been reported to indu...

Little is known about the role of the tumor suppressor gene phosphatase and tensin homolog deleted on chromosome 10 (PTEN) in prostate cancer bone metastasis. To explore this, we used a pTetOn PTEN cell line in which PTEN expression was reconstituted in a PTEN-null bone metastatic human prostate cancer cell line, LnCaP-C4-2. We found that C4-2 cells selectively migrated toward conditioned mediu...

The influence of an excess of retinol on bone formation was studied by using cultures of embryonic-chick calvaria. Retinol decreased collagen synthesis in a dose-dependent manner, non-collagenous protein synthesis being relatively unaffected. Collagen synthesis was significantly inhibited after 24 h of culture with retinol and was progressively decreased, compared with control cultures containi...

Acollagenase inhibitor was identified in the culture medium of mouse calvaria after separation from mouse bone collagenase by Sephadex G-200 gel filtration equilibrated with 6M urea in Tris buffer. The simultaneous synthesis of both collagenase and inhibitor by mouse calvaria in tissue culture was consistent with the resuls of our previous study using achick bone culture system and otehr recent...

In this work the biocompatibility of two vanadium-free Ti–Al–Nb alloys, Ti–15Al–33Nb and Ti–21Al–29Nb, was evaluated and compared to that for commercially pure titanium (CP Ti) and alumina (Al2O3). Fine particles were milled from sheet-processed material and implanted onto mice calvaria using an established animal model. Various stains, including methylene blue/acid fuchsin, TRAcP, and immunohi...

The formation of cranial bone requires the differentiation of osteoblasts from undifferentiated mesenchymal cells. The balance between osteoblast recruitment, proliferation, differentiation and apoptosis in sutures between cranial bones is essential for calvarial bone formation. The mechanisms that control human osteoblasts during normal calvarial bone formation and premature suture ossificatio...

Previously our laboratory documented increases in calvaria bone volume and thickness in mice exposed to 15 days of spaceflight aboard the NASA Shuttle mission STS-131. However, the tissues were not processed for gene expression studies to determine what bone formation pathways might contribute to these structural adaptations. Therefore, this study was designed to investigate both the structural...

considering the importance of bone augmentation prior to implant placement in order to obtain adequate bone quality and quantity, many studies have been conducted to evaluate different techniques and materials regarding new bone formation. in this study, we investigated the bone healing capacity of two different materials deproteinized bovine bone mineral (dbbm with the trade name of bio-oss) a...

Karsinoma tiroid folikuler merupakan subtipe kedua tersering yang umumnya bermetastasis ke tulang. Beberapa teknik pencitraaan dapat digunakan untuk mendeteksi adanya metastasis. Kami melaporkan kasus suspek metastasis karsinoma kemudian dinyatakan sebagai limfoma Wanita berusia 33 tahun dirujuk mendapatkan terapi radiotiroablasi menggunakan NaI 131. Pasien mengeluh suara serak, kadang masih te...