1010-7940/$ see front matter Q 2005 Elsevier B.V. All rights reserved. doi:10.1016/j.ejcts.2005.04.045 * Tel.: C31 71 5262348; fax: C31 71 5248284. E-mail address: [email protected]. no harvesting injury of the LITA reported, the pathophysiological basis was either a congenital anomaly or atheroma. A GEA to LAD and restrictive mitral annuloplasty resulted in a good outcome. European Journal of C...

HE genetic structure of Streptomyces coelicolor A3 (2), the only strain among the actinomycetes for which a genetic map has been constructed, was studied in detail by HOPWOOD (1967) and SERMONTI (1 969). For genetic analysis, HOPWOOD and SERMONTI utilized the capacity of mutant strains of A3 (2) to undergo genetic recombination. To enlarge the possibilities of genetic analysis of the actinomyce...

With the completion of genome sequences of major model organisms, increasingly sophisticated genetic tools are necessary for investigating the complex and coordinated functions of genes. Here we describe a genetic manipulation system termed ‘‘genomic engineering’’ in Drosophila. Genomic engineering is a 2-step process that combines the ends-out (replacement) gene targeting with phage integrase ...

Six mature geldings of light horse breeds (557 ± 37 kg) were randomly assigned to a nontoxic endophyte-infected tall fescue hay (n = 3) or pasture treatment (n = 3) in a crossover design with 14-d periods to estimate DMI with alkane markers and to compare DMI of hay and pasture. When fed pasture, horses were housed in stalls from 0700 to 1300 h daily with access to water and then grazed pasture...

Bacteriophage C31 encodes an integrase, which acts on the phage and host attachment sites, attP and attB, to form an integrated prophage flanked by attL and attR. In the absence of accessory factors, C31 integrase cannot catalyse attL x attR recombination to excise the prophage. To understand the mechanism of directionality, mutant integrases were characterized that were active in excision. A h...

Human alpha defensins are a class of antimicrobial peptides with additional antiviral activity. Such antimicrobial peptides constitute a major part of mammalian innate immunity. Alpha defensins contain six cysteines, which form three well defined disulfide bridges under oxidizing conditions. Residues C3-C31, C5-C20, and C10-C30 form disulfide pairs in the native structure of the peptide. The ma...

Germ-line transformation via transposable elements is a powerful tool to study gene function in Drosophila melanogaster. However, some inherent characteristics of transposon-mediated transgenesis limit its use for transgene analysis. Here, we circumvent these limitations by optimizing a C31-based integration system. We generated a collection of lines with precisely mapped attP sites that allow ...