UNLABELLED Ceramide is an important signaling molecule that is typically generated via sphingomyelinase (SMase)-mediated sphingomyelin (SM) hydrolysis. Although diverse forms of renal injury elicit ceramide accumulation, the molecular determinants of this change and its contribution to tissue damage are poorly defined. The present study uses iron (Fe/hydroxyquinoline)-mediated injury of culture...

The smallest fullerene C20 with positive electron affinity is considered to be a new organic nano-electronic material. The binding structures and electronic states of lithium ions and atoms (Li+ and Li) trapped on the surface of C20 have been investigated by means of density functional theory (DFT) calculation to elucidate the nature of their interaction. It was found that a Li+ can bind to onl...

Abstract Polyether ionophores, with >120 molecules belonging to this group, represent a class of naturally-occurring compounds that exhibit broad range pharmacological properties, including promising activity towards variety parasites. In context, salinomycin (SAL) seems be interesting, as ionophore has been found active against parasites are responsible for number human and animal diseases. On...

Abnormal lipid metabolisms are closely associated with cancers. In this study, mass spectrometry was employed to in situ investigate the associations of membrane lipid phenotypes of six human lung cancer cell lines (i.e., A549, H1650, H1975 from adenocarcinoma, H157 and H1703 from squamous cell carcinomas, and H460 from a large cell carcinoma) with cancer cell types and finally total 230 lipids...

AIM This study was designed to clarify differences in serum phospholipid fatty acid compositions and estimated desaturase activities between Japanese men with and without metabolic syndrome (MetS). METHODS From among 227 males, 40 to 59 years of age, excluding those receiving treatment for lipid disorders, 165 subjects (including 27 with MetS) were selected for this study. Serum phospholipid ...

diels–alder [2+4] cycloaddition products of the reaction between c20 and c4h4x2 or c2o2x2 (x = h, f, cl, ch3, nh2, no2, and oh) were studied atb3lyp level of theory with 6-31g, 6-31g(d, p) and 6-311g(d, p) basis sets. the homo–lumo gaps of kohn–sham orbitals for most of the adducts show evident increase compared with the gap value of c20, suggestive of more stability in the adducts. the thermod...

OBJECTIVES Effect of paraquat on the fatty acid composition (weight percentage) of rat lung was studied with particular reference to the change of hydroxyproline content in the course of paraquat-induced dysfunction and subsequent repair. METHODS Eight-week-old male Wistar rats were administered paraquat at 20 mg/kg body weight subcutaneously, and the wet weight, hydroxyproline content and fa...

The molecular structures of the three main haemolytic compounds (Fj1, Fj2 and Fj3) isolated from the ichthyotoxic microalgal species Fibrocapsa japonica have been investigated by NMR, LC-ESI-MS, ESI-MS-MS, IR, GC-MS and GC-HRMS methods. They are polyunsaturated fatty acids which we identified as: 6,9,12,15-octadecatetraenoic acid (OTA, C18:4omega3), 5,8,11,14,17-eicosapentaenoic acid (EPA, C20:...

Mass changes in the incorporation of linoleic (C18:2), eicosapentaenoic (C20:5) and docosahexaenoic (C22:6) acids in human blood platelet phospholipids were induced by incubating the cells and these fatty acids complexed to albumin. The remodelling of [14C]C18:2, [14C]C20:5 and [14C]C22:6 in classes, subclasses and molecular species of platelet phospholipids was studied in resting and thrombin-...