Apoptosis, programmed cell death, has a central role in developmental biology and maintaining the equilibrium of renewing tissues. A founding member Bcl-2 family regulatory proteins for apoptosis is Bcl-2, which encoded by BCL2 gene. Caspase-3 shares typical features with all caspases, including acting as crucial mediator apoptosis.

17-allylamino-demethoxy geldanamycin (17-AAG) inhibits the chaperone function of heat shock protein-90 (Hsp-90) and promotes the proteasomal degradation of its misfolded client proteins. Here, we demonstrate that treatment of the human acute myeloid leukemia HL-60 cells with 17-AAG attenuates the intracellular levels of a number of Hsp-90 client proteins, including Akt, c-Raf-1, and c-Src. Also...

Calcitriol [1 ,25-dihydroxyvitamin D3] is the natural ligand of the vitamin D receptor (VDR). Using cultured prostate cancer (PC) cell lines, LN-CaP and ALVA-31, we studied the effects of 1 ,25(OH)2-Vitamin D3 (VD3) on expression of several apoptosis-regulating proteins including: (a) Bcl-2 family proteins (Bcl-2, Bcl-XL, Mcl-1, Bax, and Bak); (b) the heat shock protein 70-binding protein BAG1L...

Excessive apoptosis of intervertebral disk cells and degeneration (IDD) is the prime cause low back pain. B-cell lymphoma 2 (Bcl-2) adenovirus E1B 19 kDa interacting protein 3 (BNIP3), a member Bcl-2 family, are involved in cell autophagy apoptosis. The roles mechanisms BNIP3 unclear. In this study, primary rat were prepared to study effect overexpression on their counting kit (CCK)-8 assay sho...

Permeabilization of the outer mitochondrial membrane that leads to the release of cytochrome c and several other apoptogenic proteins from mitochondria into cytosol represents a commitment point of apoptotic pathway in mammalian cells. This crucial event is governed by proteins of the Bcl-2 family. Molecular mechanisms, by which Bcl-2 family proteins permeabilize mitochondrial membrane, remain ...