Rhodobacter sphaeroides only showed chemotaxis towards ammonia if grown under nitrogen-limited conditions. This chemotactic response was completely inhibited by the addition of methionine sulfoximine. There was no effect of methionine sulfoximine treatment on motility or taxis towards propionate, demonstrating that the effect is specific to ammonia taxis. It is known that methionine sulfoximine...

Sulfoximines are an interesting class of compounds, which exhibit Brønsted and Lewis basicity, nucleophilicity, C−H acidity, N−H hydrogen-bond acceptor capability chirality. have received renewed interest in recent times, since incorporation a sulfoximine group can provide drug candidates with favorable properties. The synthesis N−H, α-C−H o-C−H functionalization sulfoximines currently topics c...

Kidney injury is an important side effect of the chemotherapeutic agent ifosfamide in humans. Previous studies have shown that treatment with ifosfamide reduces kidney glutathione and that the toxicity of ifosfamide is enhanced in glutathione-depleted renal tubule cells in vitro. In this study, we examined the effect of glutathione depletion on ifosfamide nephrotoxicity in vivo using rats treat...

BACKGROUND/AIMS Ethanol consumption and pathological conditions such as cirrhosis lead to a reduction of hepatic glutathione. Hepatic methionine adenosyltransferase, the enzyme that synthesizes S-adenosylmethionine, the major methylating agent, is regulated in vivo by glutathione levels. We have previously shown that nitric oxide inactivates methionine adenosyltransferase in vivo by S-nitrosyla...

The N2 fixing bacteria Klebsiella pneumoniae, Azospirillum brasilense, Rhodopseudomonas sphaeroides and Rhodospirillum rubrum, but not Azotobacter vinelandii accumulate the glutamine analogue methionine sulfoximine in the cell. In the accumulating cells methionine sulfoximine inhibits ammonium transport. Accumulation and inhibition are prevented by glutamine.

Article history: Received: 3 Nov 2011 Accepted: 22 July 2012 Available online: 18 Nov 2012 ZJRMS 2013; 15(5): 9-12 Background: 5TButhionine sulfoximine is an agent that reduces intracellular glutathione and anti-oxidant enzymes and by this means is involved in pathology of some disease. Since low glutathione level may affect embryonic development, the aim of the present study is to investigate ...

L-Methionine sulfoximine is a substrate of L-amino acid oxidase (Crotalus adamanteus), glutamine transaminase, and y-cystathionase. In the reaction catalyzed by L-amino acid oxidase, methionine sulfoximine is converted to oc-imino-y-methylsulfoximinylbutyrate, which undergoes rapid y elimination yielding methane sulfinimide and 2-imino-3-butenoic acid. Methane sulfinimide is converted to methan...

Both the adenylylated and unadenylylated forms of Escherichia coli glutamine synthetase catalyze the formation of pyrrolidone carboxylate from glutamate in the presence of ATP and divalent metal ions (Mg++ or Mn++) and in the absence of ammonia. The unadenylylated enzyme catalyzes this reaction more rapidly with Mg++ than with Mn++, while the adenylylated enzyme catalyzes it more rapidly with M...

objective(s): buthionine sulfoximine (bso) inhibits synthesis of glutathione as the main intracellular antioxidant. the aim of the present study is to investigate the effect of bso-induced oxidative stress on histological structure of testis, testosterone secretion and semen parameters. materials and methods: thirty male balb/c mice were divided into 3 groups. in control group, the mice did not...