Studies were conducted to examine the effect of substitution of serum with cystic follicular fluid in the IVM media and also to test the efficacy of cystic follicular fluid (buCFF) at 50 and 100 per cent levels for in vitro maturation of buffalo oocytes. Resutts indicated that buffalo cystic follicular fluid supplemented with PMSG possess the ability for induction of maturation of buffalo oocyt...

This study was undertaken to determine whether fully grown oocytes isolated from the early antral follicles (2-8 mm size) of frozen-thawed buffalo ovaries are viable and can be rescued to undergo maturation, fertilization, and embryo development in vitro. Ovaries were cryopreserved just after being collected during slaughter from the local abattoir using in situ oocyte (ISO) cryopreservation. I...

Jamil H., H.A. Samad, N.U. Rehman, Z.I. Qureshi, L.A. Lodhi: In vitro Maturation and Fertilization of Riverine Buffalo Follicular Oocytes in Media Supplemented with Oestrus Buffalo Serum and Hormones. Acta Vet. Brno 2007, 76: 399-404. Effects of two maturation media (TCM-199 and Ham’s F-12) with and without the addition of oestrus buffalo serum (OBS) and hormones (FSH, LH, E2) on the maturation...

Leptin, the 16-kDa product of the obese (ob) gene, primarily secreted from adipose tissue, has been implicated to play an important role in the regulation of food intake and energy expenditure. This study investigated protective effect of leptin on trichostatin A-induced apoptotic on in vitro maturation ratio of buffalo oocytes. Ovaries were collected from abattoir and were transported...

Studies were conducted to compare viability of immature buffalo oocytes vitrified in different types of cryoprotectants on the post-thaw morphological appearance, the in vitro maturation and developmental competence of buffalo oocytes. The vitrification solution (VS) consisted of Dulbecco’s phosphate buffered saline (DPBS) supplemented with 0.5 M sucrose, 0.4% bovine serum albumin (BSA) and dif...

In the present study, the response of Nili Ravi buffalo oocytes to recombinant human follicle stimulating hormone (rhFSH) (Organon) on meiotic maturation in vitro was examined. Oocytes were matured in vitro in medium containing either 0 or 0.05 IU/ ml rhFSH and the stage of nuclear maturation recorded after 24 hours. The percentage of oocytes in the control group undergoing germinal vesicle bre...

The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium w...

The effect of supplementation of follicular fluid (FF) at 5.0% concentration in in vitro maturation (IVM) media on in vitro production of buffalo embryos was studied. Oocytes were aspirated from slaughter house derived ovaries and graded. Cultutarable quality oocytes were randomly cultured in IVM media with FF (n = 334) and without FF (n = 228) and subjected to maturation. The maturation rate, ...

This study was carried out to illustrate the ultrastructure of Egyptian buffalo oocytes through in vitro maturation (IVM) period (24 h). Selected cumulus oocytes complexes (COCs) were divided into three groups in relation to the time of maturation (8, 16 and 24 h >). After different maturation intervals, the matured oocytes were examined by light and transmission electron microscope (TEM). Resu...

Trichostatin A (TSA), a histone deacetylase inhibitor, has been widely used to improve the cloning efficiency in several species. This brings our attention to investigation of the effects of TSA on developmental potential of swamp buffalo cloned embryos. Swamp buffalo cloned embryos were produced by electrical pulse fusion of male swamp buffalo fibroblasts with swamp buffalo enucleated oocytes....