Nuclear-mitochondrial incompatibilities may be responsible for the development failure reported in embryos and fetuses produced by interspecies somatic cell nuclear transfer (iSCNT). Herein we performed xenooplasmic transfer (XOT) by introducing 10 to 15% of buffalo ooplasm into bovine zygotes to assess its effect on the persistence of buffalo mitochondrial DNA (mtDNA). Blastocyst rates were no...

The present study was designed to examine the influence of oocyte quality, culture media and gonadotropins on cleavage rate and development of in vitro fertilized buffalo embryos. Three experiments were conducted. In experiment 1, oocytes were classified by number of cumulus cell layers and morphology of the ooplasm as good, fair or poor. Oocytes were cultured for IVM, IVF and IVC in CR1aa medi...

Background: There is a considerable numbers of buffalo (Bubalus bubalis) in Iran which have an important role in rural life. The reproductive performance of buffalo is lower than cow. Co-culture with somatice cells improved the maturation rate of most of the species. The aim of the present study was to evaluate the effect of bovine cumulus co-culture on post fertilization development of buffalo...

Assisted reproductive technologies such as artificial insemination (AI) and multiple ovulation and embryo transfer (MOET) have been introduced to overcome reproductive inefficiencies and accelerate genetic gain. However, MOET in water buffalo is compromised by the poor ovulatory response of this animal species to hormonal treatment (Karaivanov 1986; Karaivanov et al. 1990; Misra et al. 1990; Cr...

Background: The objectives of a series of studies undertaken was to improve farm animal reproductive efficiency in terms of embryo production efficiency, conception rate and litter size by immunoneutralization of inhibin in conjunction with other conventional reproductive techniques. Materials and Methods: A recombinant porcine inhibin alpha-subunit mature peptide was produced by mass scale and...

The effect of supplementation of recombinant LIF in the embryo culture medium, effect of oviductal epithelial cells and Vero cells co-culture on subsequent embryo development were studied during in vitro culture of buffalo embryos. Oocytes collected from the slaughter house ovaries, were matured and fertilized in vitro. The presumptive zygotes were cultured in four different groups as control-g...

Conventional buffalo semen freezing studies are limited in Anatolian buffaloes, which overly sensitive to exogenous stimulation. The present study's object was determine the main features of obtained by artificial vagina method for first time. A total number 150 ejaculates were collected from three Buffalo bulls (app. 4 years age). mean pH, volume and concentration found 6.63±0.15, 1.61±0.5 ml,...