The objective of this study was to evaluate the effect of reducing sperm numbers per insemination dose on fertility of cryopreserved buffalo bull semen. For this purpose, semen was collected at weekly intervals from a Nili-Ravi buffalo bull (Bubalus bubalis) using an artificial vagina in two batches. The ejaculates were split-sampled and diluted at 37°C with tris-citric acid extender having 15x...

The objective of the present study was to determine the effect of non-enzymatic antioxidants (vitamin C or E) in tris-citric acid buffer (TCA) on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Split pooled buffalo bull ejaculates were diluted in TCA egg yolk glycerol extender containing either vitamin C (TCAC), vitamin E (TCAE) or without antioxidant (TCAN) at 37°C. Extended s...

This study was designed to compare the effect of straw size (0.25 vs. 0.5 ml) and thawing time (30 vs. 60 sec) on the quality of cryopreserved buffalo bull semen. Sperm motility, plasma membrane integrity and viability were higher (p ≤ 0.05) in 0.25 ml than 0.5 ml straw, thawed at 37°C either for 30 or 60 sec. In conclusion, cryopreservation of buffalo semen in 0.25 ml straw resulted in a highe...

AIM The present study was conducted to investigate the effect of species, breed and age on bacterial load in fresh and frozen semen of Cattle and Buffalo bull. MATERIALS AND METHODS Present study covered 56 cow and 10 buffalo bulls stationed at Central Semen Station Anjora, Durg (Chhattisgarh). Impact of breeds on bacterial load in semen was assessed using six breeds of cattle viz. Sahiwal, G...

The aim of the present study was to evaluate the effects of different seasons on the morphometric testicular parameters and the respective epididymal sperm parameters. Total numbers of 104 scrota of pubertal buffalo bull (24-48 months) include their contents were provided from the local slaughterhouse. Scrotal circumference was measured on its place before dissection. Morphological parameters o...

The present study was designed to compare fertilization rates following oocyte exposure to spermatozoa from different buffalo bulls, using three sperm preparation methods i.e. percoll density gradient, swim-up (modified Ca free Tyrode’s medium and TALP medium) and sodium citrate washing prior to co-incubation with oocytes. Buffalo oocytes were aspirated from ovarian follicles within 1.5 to 2 ho...

The present study was conducted to determine the correlation of hypo-osmotic swelling test with conventional semen evaluation parameters of fresh semen collected from two Nili-Ravi buffalo and two Sahiwal cow bulls. A total of 10 pooled samples (each comprising two consecutive ejaculates) from each bull were collected. Each semen sample was divided into two parts. One part was used for the eval...

This srudy was conducted to investigate the effect of washing on motility, liveability (hours) and absolute index of liveability of buffalo bull spermatozoa at 37°C. Five pooled (1st and 2nd ejaculate) samples from each of the two buffalo bulls were collected. Each pooled ejaculate was divided into five aliquots (A, ro E). Of these, B to E were centrifuged at 3000 rpm for 15 minutes. The semina...