In order to elucidate the correlation of temperature and the effect of gibberellin spray on the development of flower-buds in Cryptomeria japonica cultivar Kumotooshi, experiments were done in controlled environmental condition during 1970-1971. The following points were observed: 1. The male flower-buds were formed maximum in the temperature range of 25°C-30°C and their formation decreased wit...

The mouse and rat have been shown to have many taste buds on the incisive papillae of the hard palate. In the rat incisive papilla, taste buds were more numerously observed than in the mouse. They were most densely distributed in both animals on the anteromedial wall of the lower part of the nasopalatine ducts. The taste buds on the mouse incisive papilla appeared at 4 or 5 days of age, and aft...

Sections of tissues containing lingual and extra-lingual taste buds were evaluated with monoclonal antibodies against cytokeratins. In the caudal third of the rat's tongue, keratin 20 immunoreactivity was restricted to taste buds, whereas keratins 7, 8, 18, and 19 were expressed in vallate and foliate taste buds and in cells of salivary ducts that merge with these taste epithelia. Hence, antibo...

close-up, the flower buds of the weed Arabidopsis thaliana appear almost animal-like. The scanning electron micrographs above show a whole inflorescence (top right) and two individual flower buds in detail. The bud of a Columbia wild-type plant shown at bottom right has an oval shape and is tightly closed — as buds normally remain until the anther matures inside. By contrast, the bud on the lef...

Brain-derived neurotrophic factor and neurotrophin-4 are required for normal taste bud development. Although these neurotrophins normally function via the tyrosine kinase receptor, trkB, they also bind to the pan-neurotrophin receptor, p75. The goal of the present study was to determine whether the p75 receptor is required for the development or maintenance of a full complement of adult taste b...

ATP and its metabolites are important for taste signaling in taste buds, and thus a clearance system for them would play critical roles in maintenance of gustatory function. A previous report revealed that mRNAs for ecto-5'-nucleotidase (NT5E) and prostatic acid phosphatase (PAP) were expressed by taste cells of taste buds, and NT5E-immunoreactivity was detected in taste cells. However, there w...

BACKGROUND Chrysanthemum morifolium is an important floral crop that is cultivated worldwide. However, due to a lack of genomic resources, very little information is available concerning the molecular mechanisms of flower development in chrysanthemum. RESULTS The transcriptomes of chrysanthemum vegetative buds, floral buds and buds were sequenced using Illumina paired-end sequencing technolog...

Efforts to unravel the mechanisms underlying taste sensation (gustation) have largely focused on rodents. Here we present the first comprehensive characterization of gene expression in primate taste buds. Our findings reveal unique new insights into the biology of taste buds. We generated a taste bud gene expression database using laser capture microdissection (LCM) procured fungiform (FG) and ...

In mammals, taste buds arise from local epithelium and are maintained by continuous cell renewal (Beidler and Smallman, 1965; Farbman, 1980; Delay et al., 1986; Stone et al., 1995). The life span of taste cells is estimated to be ~10 days. Denervation causes the degeneration of taste buds, indicating that the taste nerves trophically maintain the taste buds. It is assumed that cell proliferatio...

Axillary buds of intact pea seedlings (Pisum sativum L. cv Alaska) do not grow and are said to be dormant. Decapitation of the terminal bud promotes the growth of these axillary buds, which then develop in the same manner as terminal buds. We previously showed that unique sets of proteins are expressed in dormant and growing buds. Here we describe the cloning, sequencing, and expression of a cD...