Brucellosis caused by various species of the genus Brucella is one most important zoonotic diseases global importance with veterinary, public health, and economic concerns. The study aimed to standardize IgM IgG-based iELISA detect anti-Brucella antibodies for serodiagnosis acute chronic human brucellosis. test was standardized using 1:320 dilution smooth lipopolysaccharide (sLPS) antigen from ...

Aim: To investigate the preventive and therapeutic potential of brucella phage in experimentally infected mice with Brucella abortus (strain 544). Materials and Methods: Three groups of mice each containing 6 individuals were infected with Brucella abortus (strain 544). A preventive group was infected with Brucella abortus (strain 544), 48 hrs before phage treatment. A therapeutic group was pha...

Brucella contaminates people with raw milk, raw dairy products and especially with cheese made from raw milk. This study has aimed to detect Brucella abortus and Brucella melitensis in fresh cheese (Coban Cheese, 100-mold), traditional Afyon Tulum Cheese (Aged Skin Bag Cheese) (50-mold) and sheep-lamb tulum (50-mold) samples provided from Afyonkarahisar province. Conventional Culture Method has...

Brucella Cu-Zn superoxide dismutase (Cu-Zn SOD) is a periplasmic protein, and immunization of mice with recombinant Cu-Zn SOD protein confers protection against Brucella abortus infection. However, the role of Cu-Zn SOD during the process of Brucella infection remains unknown. Here, we report that Cu-Zn SOD is secreted into culture medium and is translocated into host cells independent of type ...

OBJECTIVES Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis. METHODS All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all avai...

Brucella abortus is generally known to cause brucellosis in cattle and buffalo. Here, we report the draft genome sequence of Brucella abortus SKN 13, isolated from aborted cattle placenta in the area of Gujarat, India, providing precious resources for comparative genomic analyses of Brucella field strains.

Genomic Detection of Brucella Abortus in Milk obtained from Farms Lorestan Province using PCR Method

A newly isolated smooth colony morphology phage-resistant strain 8416 isolated from a 45-year-old cattle farm cleaner with clinical features of brucellosis in China was reported. The most unusual phenotype was its resistance to two Brucella phages Tbilisi and Weybridge, but sensitive to Berkeley 2, a pattern similar to that of Brucella melitensis biovar 1. VITEK 2 biochemical identification sys...

Cells in the Brucella spp. are intracellular pathogens that survive and replicate within host monocytes. Brucella maintains persistent infections in animals despite the production of high levels of anti-Brucella-specific antibodies. To determine the effect of antibody opsonization on the ability of Brucella to establish itself within monocytes, the intracellular trafficking of virulent Brucella...

Brucella species are Gram-negative, facultative intracellular bacteria that cause zoonotic brucellosis. Survival and replication inside macrophages is critical for establishment of chronic Brucella infection. Virulent smooth B. abortus strain 2308 inhibits programmed macrophage cell death and replicates inside macrophages. Cattle B. abortus vaccine strain RB51 is an attenuated rough, lipopolysa...