Low-passage rat-embryo cells were not transformed by 3-methylcholanthrene or by 5-bromo-2' deoxyuridine. However, prior treatment with bromodeoxyuridine, followed by treatment with methylcholanthrene, resulted in cell transformation about three subpassages after removal of the carcinogen. RNA-directed DNA polymerase activity could not be detected in either normal or transformed cells. However, ...

When 5-bromodeoxyuridine is fed to Drosophila larvae, a variety of morphogenic lesions are found in the hatching adults. Experiments were undertaken to determine whether 5-bromodeoxyuridine is incorporated into Drosophila DNA under the conditions which induce developmental modifications. Radioautographic techniques were used to examine the distribution of [aH lbromodeoxyuridine in Drosophila la...

HL-60 human nonlymphocytic leukemia cells undergo terminal differentiation along either the myeloid or monocytic pathway in a process previously shown to involve two sequential steps, early events leading to a precommitment state and late events leading to onset of terminal differentiation. The present report shows that bromodeoxyuridine induces the early events leading to precommitment. In thi...

5-Bromodeoxycytidine is phosphorylated to 5-bromodeoxycytidine 5'-monophosphate in extracts of cells infected with herpes simplex virus but not in extracts of uninfected cells. The conversion of 5-bromodeoxycytidine to nucleotides and its utilization for DNA synthesis in uninfected cells occurs by deamination of 5-bromodeoxycytidine to 5-bromodeoxyuridine followed by phosphorylation of 5-bromod...

Previous experiments suggest that mitotic chromosome segregation in some fungi is a nonrandom process in which chromatids of the same replicative age are destined for cosegregation. We have investigated the pattern of chromatid segregation in Saccharomyces cerevisiae by labeling the DNA of a strain auxotrophic for thymidine with 5-bromodeoxyuridine. The fate of DNA strands was followed qualitat...

Ectopic genes transferred to cells are temporally expressed, although this phenomenon has not yet been well characterized. We found that 5-bromodeoxyuridine dramatically increased transient expression of ectopic genes in human cells. This effect was elicited by adding 5-bromodeoxyuridine prior to or after transfection. No promoter specificity was observed. Real time PCR analysis showed an appro...

Chromosomal appearance of 12 Holstein bulls selected for artificial inseminations were examined bysister chromatid exchanges (SCEs). For differential staining of sister chromatids bromodeoxyuridine (BrdU)was inoculated in lymphocyte cultures. The mean, maximum and minimum number of SCE per cell weredetermined 6.8 ± 1.14, 8.3 ± 1.1 and 5.7 ± 1.5, respectively. SCE frequencies of all animals were...

Selective incorporation of 5-bromodeoxyuridine into the late replicating arm of the X chromosome of Chinese hamister cells cultured in vitro caused a selective radiosensization of the arm to ionizing radiation. Radiation damage was observed as chromosomal aberrations, and incorporation was studied by using tritiated 5-bromodeoxyuridine.