this study evaluated the antimicrobial activities of two lactic acid bacteria, lactococcus lactis subsp. lactis i23 and lactococcus lactis subsp. lactis e91, against brochothrix thermosphacta in pork meat during storage at ambient temperature (30°c) for 168 h. the lab strains and the spoilage organism were inoculated on fresh pork samples at 1×106 cfug-1. results showed about 3 log reduction in...

Anaerobically, Brochothrix thermosphacta fermented glucose primarily to l-lactate, acetate, formate, and ethanol. The ratio of these end products varied with growth conditions. Both the presence of acetate and formate and a pH below about 6 increased l-lactate production from glucose. Small amounts of butane-2,3-diol were also produced when the pH of the culture was low (</=5.5) or when acetate...

The influence of atmosphere composition on the metabolism of Brochothrix thermosphacta was studied by analyzing the consumption of glucose and the production of ethanol, acetic and lactic acids, acetaldehyde, and diacetyl-acetoin under atmospheres containing different combinations of carbon dioxide and oxygen. When glucose was metabolized under oxygen-free atmospheres, lactic acid was one of th...

In two separate experiments, the bacteriocin, nisin, was incorporated into a commercially available meat binding system (Fibrimex) and applied to meat surfaces as a way of inhibiting the meat spoilage organism, Brochothrix thermosphacta during extended refrigerated storage. In experiment 1, pre-rigor lean beef carcass tissue (BCT) was inoculated with B. thermosphacta, left untreated (U), treate...

In the present study, a multiple approach was used to characterize Malpighia punicifolia extract and to evaluate its inhibitory activity against several meat spoilage bacteria. First, volatile fraction, vitamins and phenolic compounds of the extract obtained by supercritical fluid extraction were determined by GC-MS and HPLC. Then, the antimicrobial action of the extract was in vitro evaluated ...

Sterilized, lean and adipose beef carcass tissues were inoculated with Brochothrix thermosphacta, left untreated (U), or treated with 100 μg/ml nisin (N), calcium alginate (A), or 100 μg/ml nisin immobilized in a calcium alginate gel (AN). Treated tissues were aseptically processed into ground beef and populations of B. thermosphacta and nisin activity were determined during refrigerated storag...