Background: Since the sheep ‘Dolly’ was successfully cloned by somatic cell nuclear transfer (SCNT), the inefficiency of SCNT cloning and abnormality of cloned animals have been a focus of considerable controversy. Oocyte quality has been shown to contribute to poor somatic cloning and cell reprogramming efficiency with many factors affecting oocyte quality and developmental potential. It has b...

A controllable flower-like Au nanostructure array for surface-enhanced Raman scattering (SERS) was fabricated using the combined technique of the top-down approach of conventional photolithography and the bottom-up approach of electrodeposition. Au nanostructures with a mean roughness ranging from 5.1 to 49.6 nm were obtained by adjusting electrodeposition time from 2 to 60 min. The rougher Au ...

We presented a scalable fabrication method for the preparation of multilayered nano-prism vertex (NV)-tips whose dimensions can be controlled for tip-enhanced Raman spectroscopy (TERS). The NV-tip had sharp vertices (diameter ~20 nm) originated from the chemical lift-off process after the angle-grinding process, enabling high resolution imaging. TERS measurements were performed on brilliant cre...

OBJECTIVE Application of on-spot staining with brilliant cresyl blue, a supravital stain, was investigated in fine needle aspirates from breast, lymph node and thyroid. STUDY DESIGN Patients with palpable breast lumps, lymph nodes and thyroid nodules were included in the study. Brilliant cresyl blue was used to stain the smears, which were evaluated for smear adequacy as well as cytological d...

The mitochondrion is explicitly involved in cytoplasmic regulation and is the cell's major generator of ATP. Our aim was to determine whether mitochondria alone could influence fertilisation outcome. In vitro, oocyte competence can be assessed through the presence of glucose-6-phosphate dehydrogenase (G6PD) as indicated by the dye, brilliant cresyl blue (BCB). Using porcine in vitro fertilisati...

Al2O3 doped Mn3O4 nanomaterial was synthesized by low-temperature stirring method and applied as a catalyst for the degradation of organic pollutants under solar light for prospective environmental applications. The morphological and physiochemical structure of the synthesized solar photocatalyst was investigated by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), e...

objective: nutrients and antioxidants in the medium of immature oocyte have a profound effect on maturation, fertilization and development of resulting embryos. in this study the effects of melatonin as an antioxidant agent on maturation, glutathione level and expression of high mobility group box-1 (hmgb1) gene were evaluated in immature oocytes of mice stained with brilliant cresyl blue (bcb)...

Living cells of Nitella were placed in different concentrations of brilliant cresyl blue solutions at pH 6.9. It was found that the greater the concentration of the external dye solution, the greater was the speed of accumulation of the dye in the cell sap and higher was the concentration of dye found in the sap at equilibrium. Analysis of the time curves showed that the process may be regarded...

The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogenase (G6PDH) activity have shown superior quality than BCB negative (-) oocytes with high G6PDH activity, the use of a BCB staining test before IVM is still controversial. This study aimed to shed mo...