We report here the whole-genome sequences of 14 strains of Bradyrhizobium canariense, isolated from root nodules of Lupinus microanthus and Lupinus angustifolius, and 1 strain of Bradyrhizobium japonicum isolated from root nodules from Lupinus angustifolius in Algeria. These sequences add to the known diversity of this agronomically important genus.

The hemoprotein content of 17 strains of Bradyrhizobium japonicum bacteroids from field-grown plants and the corresponding strains of cultured cells was determined spectrally. The major terminal oxidases, cytochromes (cyt) aa(3) and o, were present in all strains of cultured cells. cyt aa(3) was present in significant amounts in bacteroids only in strains of DNA homology group II. cyt o appeare...

We report the draft genome sequence of Bradyrhizobium japonicum Is-1, which is incompatible with Rj2 genotype soybeans. The estimated genome size of this strain is 8.9 Mb. Genome sequence information of this strain will help to identify a causal gene for this incompatibility.

ABSTRACT The soybean chemical composition is strongly influenced by genetic factors, as well their interaction with the environment and management practices. Among environmental water deficit temperature are those that most contribute to modify of beans, especially oil protein contents. present study aimed assess effects co-inoculation Azospirillum brasilense on Two field experiments were carri...

The recent identification of the iron response regulator (Irr) in Bradyrhizobium japonicum raised the question of whether the global regulator Fur is present in that organism. A fur gene homolog was isolated by the functional complementation of an Escherichia coli fur mutant. The B. japonicum Fur bound to a Fur box DNA element in vitro, and a fur mutant grown in iron-replete medium was derepres...

The taxonomy of N2-fixing bacteria belonging to the genus Bradyrhizobium is still poorly refined. This paper presents an application of a method aiming the identification of possible new clusters within a Brazilian collection of 119 Bradyrhizobium strains showing phenotypic characteristics of B. japonicum and B. elkanii. The stability has been studied as a function of the number of restriction ...

Soybean-nodulating bradyrhizobia are genetically diverse and are classified into different species. In this study, the genetic diversity of native soybean bradyrhizobia isolated from different topographical regions along the southern slopes of the Himalayan Mountains in Nepal was explored. Soil samples were collected from three different topographical regions with contrasting climates. A local ...

Of nine Bradyrhizobium japonicum serogroup 123 strains examined, 44% were found to be restricted for nodulation by cultivar Hill. Nodulation studies with soybean isoline BARC-2 confirmed that the soybean Rj4 allele restricts nodulation by the same serogroup 123 isolates. Immunological analyses indicated that B. japonicum strains in serogroups 123 and 31 share at least one surface somatic antigen.

Reciprocal grafting experiments done using soybean plant introduction genotypes indicated that restriction of nodulation by Bradyrhizobium japonicum is determined by the genotype of the root and is dependent on plant growth temperature. Microscopic analyses indicated that the soybean plant introduction genotypes restrict nodulation of B. japonicum at symbiotic stages which occur both before and...