Extraction of serial sections of cattle hoof epidermis with solutions of calcium chloride, magnesium chloride, potassium chloride, sodium chloride, guanidine hydrochloride, ammonium sulfate, and potassium phosphate buffer (pH 7.0) at varying salt concentrations demonstrates that keratohyalin (KH) is extracted by these salts at certain molarities. Under given conditions of time and temperature, ...

Keratin from the hoof is a less explored source for making valuable products. In this paper we present the extraction of pure keratin from bovine hooves and characterized them to better address the possible exploitation of this bio-resource as an alternative material for tissue engineering applications. The keratin protein from the pulverized hooves was extracted by reduction, which was observe...

Polypeptides that form 10-nm filaments in vitro were isolated from three different bovine tissues: the viable portion of the hoof epithelium, the epithelium of the esophagus, and cultured endothelial cells derived from aorta. The seven polypeptides from hoof, the two from esophagus, and the one from endothelial cells were different with respect to mobility in SDS polyacrylamide gels and/or limi...

In an effort to increase the efficacy of topical medications for treating onychomycosis, several new nail penetration enhancers were recently developed. In this study, the ability of 10% (wt/wt) miconazole nitrate combined with a penetration enhancer formulation to permeate the nail is demonstrated by the use of a selection of in vitro nail penetration assays. These assays included the bovine h...

This work aimed to (a) characterize the microstructure and porosity of human nail and bovine hoof by mercury intrusion porosimetry and SEM image analysis, (b) study the effects of hydration and of N-acetyl-l-cysteine treatment on the microstructure of both membranes, and (c) determine whether the microstructural modifications were associated with changes in drug penetration measured by standard...

This work aimed to (a) characterize the microstructure and porosity of human nail and bovine hoof by mercury intrusion porosimetry and SEM image analysis, (b) study the effects of hydration and of N-acetyl-l-cysteine treatment on the microstructure of both membranes, and (c) determine whether the microstructural modifications were associated with changes in drug penetration measured by standard...

A narrative integrative review on the evidence for hoof trimming found 16 articles on efficacy, frequency, and associations with behavior and physiologic parameters. Review of these studies revealed (1) hoof trimming is associated with behavior and physiologic changes; (2) increasing the frequency of hoof trimming seems to decrease the incidence of hoof lesions; (3) there is limited research to...

Many performance and racehorses cannot perform to their potential because of hoof problems. The old adage “no hoof, no horse” still applies today and this article examines some of the nutritional factors that impact the hoof. There are many factors that can influence development of the hoof, and this article will discuss some of these variables that can aid the search for increased hoof growth ...

wide variety of timings and techniques has been used based on the housing, production, availability of requirements etc. this current study was done for a morphologic evaluation of the cow's digit in different trimming times. a dairy herd with 2200 milking cow, free stalls barns, average daily production of 36 liters per cow was selected. forty cows were assigned to four groups based on hoof tr...