نتایج جستجو برای: botulinum neurotoxin a

تعداد نتایج: 13434741  

2014
Karl A. Hassan Liam D. H. Elbourne Sasha G. Tetu Eric A. Johnson Ian T. Paulsen

Clostridium strains from six phylogenetic groups, C. botulinum groups I to IV, C. baratii, and C. butyricum, display the capacity to produce botulinum neurotoxin. Here, we present the genome sequence of a C. butyricum isolate, the neurotoxigenic strain 5521, which encodes the type E botulinum neurotoxin.

Abbas Hajizade, Ali Sayadmanesh, Firouz Ebrahimi, Hani Keshavarz, Mosayeb Rostamian,

Background: Botulinum neurotoxin (BoNT) complexes consist of neurotoxin and neurotoxin-associated proteins. Hemagglutinin-33 (HA-33) is a member of BoNT type A (BoNT/A) complex. Considering the protective role of HA-33 in preservation of BoNT/A in gastrointestinal harsh conditions and also its adjuvant role, recombinant production of this protein is favorable. Thus in this study, HA-33 was expr...

Journal: :Applied and environmental microbiology 1985
C Shone P Wilton-Smith N Appleton P Hambleton N Modi S Gatley J Melling

A monoclonal antibody (BA11) has been produced against Clostridium botulinum type A neurotoxin by the fusion of myeloma cells (P3 NS1/1-Ag4-1) with spleen cells from BALB/c mice immunized with botulinum type A neurotoxoid. The antibody bound specifically to botulinum type A neurotoxin, showing no cross-reactivity with types B and E botulinum toxins or with any of several other bacterial toxins ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1989
J Marsal G Egea C Solsona X Rabasseda J Blasi

The action of botulinum neurotoxin on acetylcholine release, and on the structural changes at the presynaptic membrane associated with the transmitter release, was studied by using a subcellular fraction of cholinergic nerve terminals (synaptosomes) isolated from the Torpedo electric organ. Acetylcholine and ATP release were continuously monitored by chemiluminescent methods. To catch the membr...

2017
Michael W. Peck Theresa J. Smith Fabrizio Anniballi John W. Austin Luca Bano Marite Bradshaw Paula Cuervo Luisa W. Cheng Yagmur Derman Brigitte G. Dorner Audrey Fisher Karen K. Hill Suzanne R. Kalb Hannu Korkeala Miia Lindström Florigio Lista Carolina Lúquez Christelle Mazuet Marco Pirazzini Michel R. Popoff Ornella Rossetto Andreas Rummel Dorothea Sesardic Bal Ram Singh Sandra C. Stringer

Botulinum neurotoxins are diverse proteins. They are currently represented by at least seven serotypes and more than 40 subtypes. New clostridial strains that produce novel neurotoxin variants are being identified with increasing frequency, which presents challenges when organizing the nomenclature surrounding these neurotoxins. Worldwide, researchers are faced with the possibility that toxins ...

Journal: :The Journal of Cell Biology 1979
N Hirokawa M Kitamura

Large synaptosome fractions were isolated from the cerebellar and cerebral cortices of rats and were incubated with Clostridium botulinum type A neurotoxin in vitro. The binding of the neurotoxin to the synapses was observed by electron microscopy, using the double-sandwich immunocytochemical method. Botulinum neurotoxin was preferentially bound to the presynaptic membrane in the large synaptos...

ابراهیمی, فیروز , باقری پور, محمدجواد, حاجی زاده, عباس, نظریان, شهرام,

Abstract Background and purpose: Clostridium botulinum neurotoxins are the most potent toxins that cause the life-threatening botulism syndrome in humans and animals. Researches have shown that the binding domain of the botulinum neurotoxin type E has a high immunogenicity effect that could be used as an efficient recombinant vaccine. The recombinant vaccines are not potent enough to stimulate...

Journal: :iranian journal of toxicology 0
rahim nejadrahim department of infectious diseases & dermatology, urmia university of medical sciences, urmia, iran. mohammad delirrad department of forensic medicine & toxicology, food & beverages safety research center, urmia university of medical sciences, urmia, iran.

background: foodborne botulism (fbb) is a rare paralyzing disease caused by ingestion of foods contaminated with botulinum neurotoxin (bont). it is still a health concern in many countries. this study aimed to investigate the epidemiology of fbb in west azerbaijan province, iran. methods: botulism surveillance data of the health deputy of urmia medical sciences university, northwestern iran was...

Journal: :Clinical microbiology reviews 2006
Miia Lindström Hannu Korkeala

Botulism is a potentially lethal paralytic disease caused by botulinum neurotoxin. Human pathogenic neurotoxins of types A, B, E, and F are produced by a diverse group of anaerobic spore-forming bacteria, including Clostridium botulinum groups I and II, Clostridium butyricum, and Clostridium baratii. The routine laboratory diagnostics of botulism is based on the detection of botulinum neurotoxi...

2011
Yiangos Yiangou Uma Anand William R Otto Marco Sinisi Michael Fox Rolfe Birch Keith A Foster Gaurav Mukerji Ayesha Akbar Sanjiv K Agarwal Praveen Anand

BACKGROUND There is increasing evidence that botulinum neurotoxin A may affect sensory nociceptor fibers, but the expression of its receptors in clinical pain states, and its effects in human sensory neurons, are largely unknown. METHODS We studied synaptic vesicle protein subtype SV2A, a receptor for botulinum neurotoxin A, by immunostaining in a range of clinical tissues, including human do...

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