Ten phenols were selected as natural laccase mediators after screening 44 different compounds with a recalcitrant dye (Reactive Black 5) as a substrate. Their performances were evaluated at different mediator/dye ratios and incubation times (up to 6 h) by the use of Pycnoporus cinnabarinus and Trametes villosa laccases and were compared with those of eight known synthetic mediators (including -...

Methylene blue was efficiently removed from aqueous solution by foam flotation using a rhamnolipid a biosurfactant as a dye collector. The effects of four parameters, namely, pH (1.5–11.5), frother concentration (5–65 ppm), aeration rate (2–6 L/min) and rhamnolipid to methylene blue weight ratio (0.5–6.5), on dye removal were studied and optimized using response surface methodology. Results...

Diphtheria toxin fragment A interacts with Cibacron blue in solution, although it is not retained by blue Sepharose columns. Difference spectral titration of fragment A with the dye gives a dissociation constant of the order of 10(-5) M and a 1:1 stoichiometry for the complex. In equilibrium dialysis experiments Cibacron blue behaves as a competitive inhibitor of the binding of NAD to diphtheri...

The adsorption of cationic organic dyes (methylene blue, thionine, and thiopyronine) on Qbeta bacteriophage was studied by UV-visible and fluorescence spectroscopy. The dyes have shown a strong affinity to the virus and some have been used as sensitizers for photo-induced inactivation of virus. In the methylene blue concentration range of 0.1-5 microM and at high ratios of dye to virus (greater...

of cancer with the hope of developing useful diag nostic and therapeutics agents. The aim in such investigations has been to find a dye which, in the intact animal, stains living tumor cells without staining surrounding tissue. A large number of acid dyes, especially in the azo series, has been rather intensively investigated for such a purpose. From these investigations there has resulted gen ...

In 1969 it was dem onstrated1 that pyruvate kinase binds to Blue Dextran 2000. In a study of the mechanism of this absorption it could be shown that the binding of the enzyme is due to an inter­ action of the enzyme with the blue dye of Blue Dextran 2000 and not with the dextran itself. This property was used for the purification of pyruvate kinase of yeast1 and later on also applied to the ex­...

Yeast hexokinase, a homodimer (100 kDa), is an important enzyme in the glycolytic pathway. Although Cibacron Blue 3G-A (Reactive Blue 2) has been previously shown to inactivate yeast hexokinase, no comprehensive study exists concerning the nature of interaction(s) between hexokinase and the blue dye. A comparison of the computer-generated three-dimensional (3D) representations showed considerab...

In this study, montmorillonite clay was used as the adsorbent for the removal of Aniline Blue dye, from aqueous solution by the batch adsorption technique under different conditions. The maximum removal of aniline blue dye for initial dye concentration 20 mg/L was more than 95% at optimum conditions (pH=4.5, contact time=30 min, and adsorbent dosage=2 g/L). The data of this study were fitted wi...

STUDY OBJECTIVE To compare the relative utility of blue dye visualization with a glucose oxidase test strip method for detecting aspiration of enteral feedings. DESIGN Tracheally intubated adults were prospectively monitored for aspiration of enteral feedings. SETTING Intensive care units of two community hospitals in Michigan. INTERVENTIONS None. PATIENTS The experimental group consist...

UNLABELLED BACKGROUND This clinical study evaluated the effect of a suctioning maneuver on aspiration past the cuff during mechanical ventilation. METHODS Patients intubated for less than 48 hours with a PVC-cuffed tracheal tube, under mechanical ventilation with a PEEP ≥5 cm H2O and under continuous sedation, were included in the study. At baseline the cuff pressure was set at 30 cm H2O. ...