This protocol describes two similar methods for the extraction and methylation of fatty acids from bacterial cultures. The acid derivatization protocol (Lennen et al., 2013; Bligh and Dyer, 1959) results in the extraction and methylation of all fatty acids, both free and bound, from a bacterial culture, while the base derivatization protocol (Lennen and Pfleger, 2013) captures only bound (phosp...

A. Introduction B. Storage of Tissues and Preliminary Treatments prior to Extraction C. Extraction of Tissues with Solvents D. Removal of Non-Lipid Contaminants from Extracts E. Artefacts of Extraction Procedures F. Practical Extraction Procedures 1. Some general comments 2. The "Folch" procedure 3. The Bligh and Dyer method 4. Extraction of plant tissues G. Some Special Cases 1. Acyl-coenzyme ...

O óleo extraído da semente de amendoim tem propriedades benéficas á saúde por ser antioxidante, antinflamatório e prevenir doenças coronárias. Apesar desta qualidade, o processamento armazenamento inadequado pode causar degradações como as oxidações lipídicas devida sua poli-insaturação. trabalho teve objetivo avaliar características físico-químicas do comercial amendoim. A extração análises ín...

The overconsumption of energy results in the depletion fossil fuels. Generally, biodiesels are produced from wastes animal fats and vegetable oils. In this study, we have tried to produce biodiesel both wild strain ion beam mutated compared concentration lipids strains their properties. Lipids were extracted microbes using Bligh Dyer method analyzed gas chromatography mass spectrophotometry (GC...

Increasing interest in receptor-regulated phospholipase C and phospholipase D hydrolysis of cellular phosphatidylcholine motivates the development of a sensitive and simple assay for the water-soluble hydrolytic products of these reactions, phosphocholine and choline respectively. Choline was partially purified from the methanol/water upper phase of a Bligh & Dyer extract by ion-pair extraction...

Two different relatively simple, commercially available supercritical fluid extractors (SFE), Leco and Foss-Tecator, were tested for the determination of total fat content in meat and meat products. The fatty acid composition in meat and meat products was also determined after the Foss-Tecator extraction in an aliquot of the extract. Total fat was determined by weighing after the different extr...

Two new methods, the modified International Dairy Federation and the modified Tiron methods, were developed for the determination of hydroperoxides in liposomes. Interferences of alpha-tocoferol and egg-phosphatidylcholine (EPC) required a solid-phase extraction before the analysis to eliminate alpha-tocoferol and a fluid-fluid extraction based on the solvent triangle of Bligh and Dyer to separ...