UNLABELLED OBJECTIVES This study sought to evaluate the outcome of fresh and vitrified-warmed cleavage-stage and blastocyst-stage embryo transfers in patients undergoing ART treatment within an ethnic Chinese population. STUDY DESIGN We compared the clinical results of embryo transfer on the 3rd (cleavage stage) or 5th (blastocyst stage) day after oocyte retrieval, including clinical pregn...

introduction: this study was started to improve in vitro blastocyst development and avoiding of blastocyst collapsment (no visibie cavity, returning of hatching blastocyst into zona pellucida, and tendency to degeneration). materials and methods: 24hrs one - cell mouse embryos were recovered from positive plug females and cultured in following canditions: experiments 1,2: during first 48hrs nmr...

Soluble proteins were studied in the developing rabbit blastocyst by using a micro-disc electrophoresis technique. The tissues of the blastocyst (in later stages separated into the embryoblastic and trophoblastic parts) were analysed, and their protein patterns were compared with those of uterine secretion and blastocyst fluid. A protein pattern with more than 20 protein fractions was found in ...

abstractl-carnitine (lc) is an antioxidant with the ability to promote the growth in vitro embryo.objective: the goal was to evaluate the effect of lc on some indicators of embryo development and blastocyst quality including zona pellucid (zp) thickness, the hatching of blastocysts and their cell numbers.materials and methods: mouse embryos were randomly divided into five groups and incubated w...

BACKGROUND The selection of blastocyst warmed for transfer is based on pre-freeze morphology in vitrified-warmed single blastocyst transfer cycles. But, it is controversial which parameter of blastocyst morphology most closely related to the clinical outcomes. OBJECTIVE To estimate the effect of blastocoele expansion, trophectoderm (TE) morphology grade, and inner cell mass (ICM) morphology g...

the effect of modified vitrification was assessed on cellular development capability in mouse embryos cultured in vitro. in this study, 466 embryos (from zygote to morula stages) were vitrified then thawed embryos have been incubated for in vitro farther development up to blastocyst stage. also, vitrification and thawing procedures were the same for all experimental groups. mouse different embr...

background: matrigel (extracellular matrix) can improve the growth of many cell types in vitro. objective: the aim of the present study was to determine the effect of matrigel on the development of 2-4 cells human embryos in culture. material and methods: surplus 2-4 cells human embryos, resulting from icsi, were divided into two groups (control and test). quality of embryos in both groups was ...