Recent advances in DNA sequencing have resulted in several terabytes of DNA sequences. These sequences themselves are not informative. Biologists usually perform comparative analysis of DNA queries against these large terabyte databases for the purpose of developing hypotheses pertaining to function and relation. This is typically done using software on a general multiprocessor. However, these ...

Tasks The blastn and blastp applications have a –task option. This option sets the parameters (e.g., word-size or gap values) to typical values for a specific type of search. For example, the “megablast” task is optimized for intraspecies comparison as it uses a large word-size, whereas “blastn” is better suited for interspecies comparisons with a shorter word-size. These tasks resemble the “Pr...

To provide a fresh and less-biased global set of analyses, large-scale comparative DNA sequence alignments between the chimpanzee and human genomes were performed with the BLASTN algorithm. One group of experiments was conducted with query and subject low-complexity sequence masking enabled while the second set had masking parameters disabled. Each group of sub-experiments tested fifteen combin...

The growing interest in genomic research has caused an explosive growth in the size of DNA databases making it increasely challenging to perform searches on them. In this paper, we proposed an index structure called the ed-tree for supporting fast and effective homology searches on DNA databases. The ed-tree is developed to enable probe-based homology search algorithms like Blastn which generat...

In the scope of the present work, four SuperSAGE libraries have been generated, using bulked root tissues from four drought-tolerant accessions as compared with four bulked sensitive genotypes, aiming to generate a panel of differentially expressed stress-responsive genes. Both groups were submitted to 24 hours of water deficit stress. The SuperSAGE libraries produced 8,787,315 tags (26 bp) tha...

Basic Local Alignment Search Tool (BLAST) is a standard computer application that molecular biologists use to search for sequence similarity in genomic databases. BLASTN, a version of BLAST specifically designed for DNA sequence searches i.e., it will find the similarities between the query sequence and the subject sequence. This similarity is to understand the function and evolutionary history...

BLAST (basic local alignment search tool) is one of the most popular sequence analysis tools used by molecular biologists. It is designed to efficiently find similar regions between two sequences that have biological significance. Because the genomic databases size is growing rapidly, when performing a complete genomic database search of the computation time of BLAST, it is continuously increas...

UNLABELLED BlastAlign uses NCBI blastn to build a multiple nucleotide alignment and is intended for use with sequences that have large indels or are otherwise difficult to align globally. The program builds a matrix representing regions of homology along the sequences, from which it selects the 'most representative' sequence and then extracts the blastn query-anchored multiple alignment for thi...

We present and validate BlastR, a method for efficiently and accurately searching non-coding RNAs. Our approach relies on the comparison of di-nucleotides using BlosumR, a new log-odd substitution matrix. In order to use BlosumR for comparison, we recoded RNA sequences into protein-like sequences. We then showed that BlosumR can be used along with the BlastP algorithm in order to search non-cod...

\ Abstract: BLAST (Basic Local Alignment Search Tool) is one of the most popular sequence analysis tools used by molecular biologists. It is designed to efficiently find similar regions between two sequences that have biological significance. However, because the size of genomic databases is growing rapidly, the computation time of BLAST, when performing a complete genomic database search, is c...